Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Goat Immunogen Purified Mouse IgG (H&L) (>98%) Product Concentration 1.2 mg/ml Formulation This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative. Storage and Handling This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Biotin conjugate containing glycerol should be stored at -20°C in a manual defrost freezer. Expiration Date One year from date of reconstitution Country of Origin USA Shipping Next Day Ambient Working Concentration The suggested working dilutions for the following applications are: ELISA: 1:20,000 – 1:400,000. WB: 1:20,000 – 1:400,000. IHC: 1:500 – 1:5000. FC: 1:200 – 1:1,000. IF: 1:200 – 1:1,000. NOTE: Titration of the reagent is recommended for optimal performance for each application. Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended. Each investigator should determine their own optimal working dilution for specific applications. RRIDAB_2892949 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Whole Molecule Anti-Mouse IgG (H&L) recognizes Mouse IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity. Antigen DetailsProtein PubMed |
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