Anti-Human CD3 – DyLight® 594

Anti-Human CD3 – DyLight® 594

Product No.: C2027

[product_table name="All Top" skus="C2027"]

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Clone
UCHT-1
Target
CD3
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG1 κ
Applications
FC
,
IF

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 594 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 594 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Red Laser (590 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
IF
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone UCHT-1 recognizes an epitope on human CD3ε.
Antigen Distribution
CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Ligand/Receptor
Peptide antigen bound to MHC
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. van Dongen, J. et al. (1988) Blood 71:603
2. Meuer, SC. et al. (1983) Nature 303:808
3. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329
4. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
Flow Cytometry
IF
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.