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Anti-Human CD3 (CD3) Allophycocyanin (APC)

Monoclonal Antibody


Prod. No.:C309
Clone:UCHT-1
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD3 (Clone UCHT-1) recognizes Human CD3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein.1 The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 antigen/T-cell receptor complex (TCR).2

Antigen Distribution:
The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL cells.

Reporter Molecule:
Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: This antibody can be used at a concentration of ≤ 0.2 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume). Each investigator should determine their own optimal working dilution for specific applications.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Leukocyte Typing VI (1996) Oxford University Press
2. van Dongen, J. J. M. et al. (1988) Blood 71:603
3. Meuer, S. C. et al. (1983) Nature 303:808
4. Beverly, P.C.L. et al. (1981) Eur. J. Immunol. 11:329
5. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
Phycobilliproteins are protected under the following patents: (U.S. patents No. 4,520,110 and 4,542,104, European patent no. 76695, Canadian patent No. 1,179,942 and Australian patent No. 548,440).
Related Products
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PN:
Anti-Human CD3 Purified (Leinco Prod. No.: C105)
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