ABTS One Component HRP Microwell Enhancer

The high quality of the enhancer can be preserved by storing at room temperature (22°C - 28°C) in the original container.

Stable for a minimum of 36 months from the manufactured date when properly stored at room temperature, in the original container.

As with other Horseradish Peroxidase substrates, ABTS reactivity can be improved with the addition of a chemical enhancer. ABTS 1 Component HRPO Microwell Enhancer is optimized for use with ABTS HRPO Microwell Substrate One Component "Ready Use" (Leinco Prod. No.: A202). When ABTS 1 Component HRPO Microwell Enhancer is used, ABTS reactivity can be increased from 50% to 150%. See the graph below for an example of the resulting increase in reactivity. This could be useful for improving assay sensitivity or to help conserve assay components. ABTS 1 Component HRPO Microwell Enhancer is not recommended for membrane or immunohistochemical applications that require a precipitation reaction product.

ABTS 1 Component HRPO Microwell Enhancer is supplied as an 11X concentrate. For microwell applications, add 1 mL of ABTS 1 Component HRPO Microwell Enhancer to 10 mL of ABTS HRPO Microwell Substrate One Component "Ready Use" (Leinco Prod. No.: A202). Mix gently for approximately 1 minute.

Add 100 μl of the enhanced substrate solution to each well of the 96 well ELISA plate. Once a soluble blue-green reaction product develops, the plate can be read at 405 nm to 410 nm. The absorbance values of the sample should be monitored so that a linear curve can be plotted. The substrate reaction can be stopped by addition of 100 μl of a stop solution such as 1% sodium dodecyl sulfate (SDS) or 405/410 nm Stop Reagent for ABTS Microwell (Leinco Prod. No.: A204). OD values should be read at 405 nm to 410 nm with or without the addition of stop solution. Estimated incubation times for substrate range from 15 to 30 minutes.