Anti-6 His [Polyclonal] – FITC Conjugate

Anti-6 His [Polyclonal] – FITC Conjugate

Product No.: 18812F

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Target
6 His
Product Type
Polyclonal
Isotype
Whole IgG
Applications
ICC
,
IF
,
IHC

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Select Product Size
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Antibody Details

Product Details

Host Species
Goat
Immunogen
6-His (HHHHHH) conjugated to KLH
Product Concentration
1.0 mg/ml
Formulation
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
State of Matter
Liquid
Product Preparation
Purified by peptide immuno-affinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at 2-8°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco


Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunofluorescence: use at a dilution of 1:100-1:400.

These are recommended dilutions.

End users should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.
Background
A polyhistidine-tag consists of at least six histidine (His) residues, often at the N- or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Antigen Details

Research Area
Epitope Tags

References & Citations

ICC
IF
IHC
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.