Anti-Acetylated Lysine [Clone 7F8]
Anti-Acetylated Lysine [Clone 7F8]
Product No.: 56455
Data
Immunohistochemistry analysis using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8 (56455). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody (56455) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.
Western Blot analysis of acetylated lysine showing detection of Acetylated Lysine protein using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8 (56455). Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody (56455) at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker. - -
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Antibody DetailsProduct DetailsReactive Species All Host Species Mouse Immunogen Acetylated KLH Product Concentration Lot Specific Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and
thawing. Regulatory Status For in vitro investigational use only. Not for
use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1ug/mL.
These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Acetylated chicken erythrocyte histones. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes proteins containing
acetylated lysine residues. It does not react
with non-acetylated lysine residues. Background Acetylation is a common post-translational modification of lysine. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs). Post- translational acetylation and deacetylation of lysine have been recognized as intracellular signals that are critical in regulating gene transcription, cell cycle progression, apoptosis, DNA repair, and cytoskeletal organization. Regulation of protein acetylation is impaired in some cancers and polyglutamine diseases, and HDACs are promising targets for anti-cancer drugs currently under investigation. Research Area Neuroscience References & CitationsTechnical Protocols ICC IF    Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
