Data
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EndoPDI Monoclonal Antibody, Clone 2E7/7 (56458). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EndoPDI Monoclonal Antibody (56458) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X. - -
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Synthetic peptide corresponding to aa 52-63 (ADGEDGQDPHSK) of human EndoPDI (accession no. NP_001139021). Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles.
Regulatory Status For in vitro investigational use only. Not
intended for diagnostic or therapeutic
applications. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1ug/mL. A band of ~48kDa is detected.
Positive control: heat-shocked HeLa cell lysate. These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human
EndoPDI. Background Endothelial protein disulfide isomerase (EndoPDI) is a thiredoxin member of the protein disulfide isomerase family of chaperones. This enzyme is localized in the endoplasmic reticulum in primary endothelial cells but colocalizes with nucleoli in the nuclei of breast, colon, and kidney cancer cells. EndoPDI mRNA and protein expression is iduced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia. Function Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast (By similarity). {ECO:0000250}. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & CitationsTechnical Protocols ICC IF    Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
