Anti-HGAL Antibody (34044)

Anti-HGAL Antibody (34044)

Product No.: 34044

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Clone
1H1-A7
Target
HGAL
Formats AvailableView All
Product Type
Monoclonal
Alternate Names
Germinal center B-cell-expressed transcript 2 protein, Germinal center-associated lymphoma protein, hGAL
Isotype
Mouse IgG2a
Applications
IHC
,
WB

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Data

Immunohistochemistry: use at 1-10ug/ml on paraffin-embedded tissues with heat-induced antigen retrieval in citrate buffer (10mM|pH 6.0Immunohistochemistry: use at 1-10ug/ml on paraffin-embedded tissues with heat-induced antigen retrieval in citrate buffer (10mM|pH 6.0
Immunoblotting: use at 1-10ug/ml. HGAL mRNA and protein expression in lysates of indicated cell lines. Actin served as a loading control.Immunoblotting: use at 1-10ug/ml. HGAL mRNA and protein expression in lysates of indicated cell lines. Actin served as a loading control.
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
GST-HGAL fusion protein (Accession no. AF521911)
Product Concentration
Lot Specific
Formulation
This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added.
State of Matter
Liquid
Product Preparation
Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco


Immunohistochemistry: use at 1-10ug/ml on paraffin-embedded tissues with heat-induced antigen retrieval in citrate buffer (10mM, pH 6.0, 10 minutes).

Immunoblotting: use at 1-10ug/mlHGAL mRNA and protein expression in lysates of indicated cell lines. Actin served as a loading control.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
This antibody recognizes human HGAL.
Background
The human germinal-center-associated lymphoma (HGAL) gene was originally identified from an expressed sequence tag that was associated with improved survival in patients with diffuse large B-cell lymphoma (DLBCL). Cloning and characterization of HGAL indicated that it is induced by IL-4. HGAL is a cytoplasmic protein and has been demonstrated in follicular lymphoma, Burkitt lymphoma, lymphocyte-predominant Hodgkin lymphoma, and a subset of DLBCL. HGAL expression is an important predictor of overall survival in patients with germinal center- derived tumors. HGAL protein is also found in germinal centers of normal tonsils and lymph nodes.
Function
Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation. {PubMed:17823310, PubMed:20844236, PubMed:23299888}.
NCBI Gene Bank ID
UniProt.org
Research Area
Cancer Research

References & Citations

1) Fouad-Younes S et al. 2010 Am J Surg Pathol 34: 1266-1276.
2) Natkunam Y et al. 2006 Blood 109: 298-305.
3) Natkunam Y et al. 2005 Blood 105: 3979-3986.
4) Lossos IS et al. 2003 Blood 101: 433-440.
IHC
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.