Anti-Human/Mouse HGAL – Dylight® 488

Anti-Human/Mouse HGAL – Dylight® 488

Product No.: H214

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Clone
1H1-A7
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
GCSAM, GCAT2; GCET2
Isotype
IgG2a κ
Applications
ELISA
,
IF Microscopy
,
IHC
,
IP
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Human/Mouse
Host Species
Mouse
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Excitation Laser
Blue Laser (493 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA2,
WB2, 4, 6, 7, 9
Additional Applications Reported In Literature ?
IHC2, 7,
IP4, 6, 7, 9,
IF Microscopy4
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
1H1-A7 activity is directed against human and mouse HGAL.
Background
HGAL (human germinal center-associated lymphoma) is a prognostic marker that predicts improved survival in patients with diffuse large B-cell or classic Hodgkin lymphoma1, 2, 3. HGAL protein is involved in several signaling pathways and cellular functions. HGAL decreases cell motility by interacting with F-actin and myosin II, inhibits the ability of myosin to translocate actin, and directly interacts with RhoA-specific guanine nucleotide exchange factors PDZ-RhoGEF and LARG4, 5, 6. HGAL also enhances intracellular B-cell receptor signaling by directly binding to Syk and enhancing Syk kinase activity7. Additionally, HGAL expression is upregulated after IL-4 stimulation8.

HGAL protein localizes to both the cell cytoplasm and membrane2, 4, 7, 9, with a major proportion localizing to lipid rafts9. Localization is modulated by BCR activation and is facilitated by myristoylation and palmitoylation protein modifications9. Additionally, IL-6 induces HGAL phosphorylation and relocalization to podosome-like structures4.

The anti-HGAL 1H1-A7 antibody was generated by immunizing mice with a GST-HGAL fusion protein and then fusing draining lymph node or spleen cells to K6H6B5 fusion partner hybridoma cells2. Hybridoma supernatants were screened by ELISA, subcloned, and then further screened by immunoblotting cellular lysates from stably transfected Daudi and HeLa cells expressing HGAL. 1H1-A7 is an IgG2a antibody containing a κ light chain. Human HGAL and its homologous mouse protein M17 are highly conserved but have some differences in expression pattern8. A comparison of real-time PCR and Western blotting data show a strong correlation between HGAL mRNA expression and protein abundance2.
Antigen Distribution
HGAL is expressed in germinal center (GC) lymphocytes, spleen, thymus, and many lymphoma subtypes, predominantly in GC-derived lymphomas and particularly in B-cell and Hodgkin lymphomas.

Antigen Details

Research Area
Cancer
.
Immuno-Oncology
.
Immunology
.
Oncology

References & Citations

1. Lossos IS, Alizadeh AA, Rajapaksa R, et al. Blood. 101(2):433-440. 2003.
2. Natkunam Y, Lossos IS, Taidi B, et al. Blood. 15;105(10):3979-3986. 2005.
3. Azambuja D, Lossos IS, Biasoli I, et al. Leuk Lymphoma. 50(11):1830–1836. 2009.
4. Lu X, Chen J, Malumbres R, et al. Blood. 110(13):4268-4277. 2007.
5. Jiang X, Lu X, McNamara G, et al. Blood. 116(24):5217–5227. 2010.
6. Lu X, Kazmierczak K, Jiang X, et al. FEBS J. 278(11):1922–1931. 2011.
7. Romero-Camarero I, Jiang X, Natkunam Y, et al. Nat Commun. 4:1338. 2013.
8. Christoph T, Rickert R, Rajewsky K. Int Immunol. 6(8):1203-11. 1994.
9. Lu X, Sicard R, Jiang X, et al. Blood. 125(4):649-57. 2015.
Indirect Elisa Protocol
IF Microscopy
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.