Anti-Human CD107a (LAMP-1) – Purified (PhenoCycler-Fusion (CODEX)® Ready)

Human adult adherent peripheral blood cells

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.

This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

IHC,
IF,
WB,
IP,

H4A3 activity is directed against human LAMP-1 (CD107a).

LAMP-1 primarily localizes to the periphery of the lysosome and is a constituent of the lysosome membrane. LAMP1 is ubiquitously expressed in human tissues.

Lysosomes are membrane-bound vacuoles that digest foreign materials and perform specialized autolysis functions¹. LAMP-1 is a lysosome-associated membrane glycoprotein¹ involved in lysosomal biogenesis, pH regulation, autophagy, and cholesterol homeostasis that also plays a role in natural killer (NK) cell cytotoxicity and protects NK cells from degranulation-associated damage induced by their own cytotoxic granule content². Additionally, LAMP-1 presents carbohydrate ligands to selectins and facilitates microbial infection of Lassa virus and Mumps virus via interactions with viral glycoprotein and fusion protein F, respectively. LAMP-1 is highly abundant in lysosomes and can be used as a maker to determine the amount and distribution of lysosomes³.

H4A3 was generated by immunizing Sprague Dawley rats with NIH/3T3 cells⁴. Spleen cells were fused with murine myeloma P3X653-Ag8 cells. The antibody was deposited in the Developmental Studies Hybridoma Bank when first developed.

Galaptin

P11279

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