Anti-Human CD107a (LAMP-1)

Product No.: C2521

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Clone
H4A3
Target
CD107a
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
LGP-120
Isotype
Mouse IgG1 κ
Applications
FC
,
IF
,
IHC
,
IP
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Human adult adherent peripheral blood cells
Product Concentration
0.5 mg/ml
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
State of Matter
Liquid
Storage and Handling
This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
IHC,
IF,
WB,
IP,
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
H4A3 activity is directed against human LAMP-1 (CD107a).
Antigen Distribution
LAMP-1 primarily localizes to the periphery of the lysosome and is a constituent of the lysosome membrane. LAMP1 is ubiquitously expressed in human tissues.
Background
Lysosomes are membrane-bound vacuoles that digest foreign materials and perform specialized autolysis functions1. LAMP-1 is a lysosome-associated membrane glycoprotein1 involved in lysosomal biogenesis, pH regulation, autophagy, and cholesterol homeostasis that also plays a role in natural killer (NK) cell cytotoxicity and protects NK cells from degranulation-associated damage induced by their own cytotoxic granule content2. Additionally, LAMP-1 presents carbohydrate ligands to selectins and facilitates microbial infection of Lassa virus and Mumps virus via interactions with viral glycoprotein and fusion protein F, respectively. LAMP-1 is highly abundant in lysosomes and can be used as a maker to determine the amount and distribution of lysosomes3.

H4A3 was generated by immunizing Sprague Dawley rats with NIH/3T3 cells4. Spleen cells were fused with murine myeloma P3X653-Ag8 cells. The antibody was deposited in the Developmental Studies Hybridoma Bank when first developed.

Antigen Details

Ligand/Receptor
Galaptin
NCBI Gene Bank ID
UniProt.org
Research Area
Cell Biology
.
Immunology
.
Neuroscience

References & Citations

1 Chen JW, Murphy TL, Willingham MC, et al. J Cell Biol. 101(1):85-95. 1985.
2 https://www.uniprot.org/uniprotkb/P11279/entry
3 Jensen SS, Aaberg-Jessen C, Christensen KG, et al. Int J Clin Exp Pathol. 6(7):1294-1305. 2013.
4 Hughes EN, August JT. J Biol Chem. 256(2):664-671. 1981.
5 Piccolo P, Sabatino V, Mithbaokar P, et al. Mol Genet Genomic Med. 7(9):e844. 2019.
6 Yu T, Chung C, Shen D, et al. Hum Mol Genet. 21(14):3205-3214. 2012.
7 Sudhan DR, Siemann DW. Clin Exp Metastasis. 30(7):891-902. 2013.
8 Faron M, Fletcher JR, Rasmussen JA, et al. Infect Immun. 81(8):2800-2811. 2013.
9 Fernandes MC, Flannery AR, Andrews N, et al. Cell Microbiol. 15(6):977-991. 2013.
10 Chapel A, Kieffer-Jaquinod S, Sagné C, et al. Mol Cell Proteomics. 12(6):1572-1588. 2013.
11 Del Giudice MG, Ugalde JE, Czibener C. Infect Immun. 81(3):956-964. 2013.
12 Roset MS, García Fernández L, DelVecchio VG, et al. Infect Immun. 81(2):521-530. 2013.
13 Notte A, Ninane N, Arnould T, et al. Cell Death Dis. 4(5):e638. 2013.
14 McDonough JA, Newton HJ, Klum S, et al. mBio. 4(1):e00606-12. 2013.
15 Newton HJ, Kohler LJ, McDonough JA, et al. PLoS Pathog. 10(7):e1004286. 2014.
16 Kohler LJ, Reed ShC, Sarraf SA, et al. mBio. 7(4):e01127-16. 2016.
17 Byndloss MX, Tsai AY, Walker GT, et al. mBio. 10(4):e01538-19. 2019.
18 Chong A, Starr T, Finn CE, et al. Mol Microbiol. 112(4):1270-1283. 2019.
19 Yang Z, Funke BH, Cripe LH, et al. Circ Cardiovasc Genet. 3(2):129-137. 2010.
20 Torres AG, Fabani MM, Vigorito E, et al. Nucleic Acids Res. 40(5):2152-2167. 2012.
21 Bekier ME 2nd, Wang L, Li J, et al. Mol Biol Cell. 28(21):2833-2842. 2017.
Flow Cytometry
IF
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.