Anti-Human CD25 (IL-2Rα) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C9305
Clone
7G7B6
Protein
CD25
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL-2Rα, CD25, Ly-43, p55, Tac
Isotype
Mouse
IgG2a
Applications
ELISA
,
FC
,
IF Staining
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C9305- 1mg
1.0 mg
$225.00
$175.50
In stock
Max:
Min: 1
Step: 1
C9305- 5mg
5.0 mg
$445.00
$347.10
In stock
Max:
Min: 1
Step: 1
C9305- 25mg
25 mg
$1,115.00
$869.70
In stock
Max:
Min: 1
Step: 1
C9305- 50mg
50 mg
$1,680.00
$1,310.40
In stock
Max:
Min: 1
Step: 1
C9305- 100mg
100 mg
$2,800.00
$2,184.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Recombinant human peripheral blood mononuclear cells (PBMCs)
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA
FC
WB
Other Applications Reported In Literature ?
IF
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 7G7B6 reacts with human IL-2Rα.
Antigen Distribution
IL-2Rα is expressed on activated mature T and B lymphocytes, during early stages of thymocytes development, pre-B cells, and in activated CD4+ memory T-lymphocytes.
Background
CD25 (IL-2Rα) is the 55 kDa subunit of the interleukin 2 receptor alpha chain which has been shown to play roles in lymphocyte differentiation, activation, and proliferation. IL-2Rα is a transmembrane glycoprotein comprised of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. IL-2Rα heterodimerizes with IL-2Rβ (CD122) and IL-2Rγ (CD132) and forms the IL-2 receptor complex. IL-2Rα by itself binds IL-2 with relatively low affinity, while the IL-2 receptor complex binds IL-2 with high affinity. IL-2Rα mutations cause severe immunodeficiency.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Xi, X. et al. (2016) Front. Med. 10(3): 311–319
2. Hong, X. et al.(2015) Biochim Biophys Acta. 1853(2):489-99
Elisa Sandwich Protocol
Flow Cytometry
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.