Anti-Human CD25 (IL-2Rα) – Purified

Anti-Human CD25 (IL-2Rα) – Purified

Product No.: C9310

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Clone
7G7B6
Target
CD25
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL-2Rα, CD25, Ly-43, p55, Tac
Isotype
IgG2a
Applications
ELISA
,
FC
,
IF Staining
,
IP
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Recombinant human peripheral blood mononuclear cells (PBMCs)
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA
FC
WB
Additional Applications Reported In Literature ?
IF
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 7G7B6 reacts with human IL-2Rα.
Antigen Distribution
IL-2Rα is expressed on activated mature T and B lymphocytes, during early stages of thymocytes development, pre-B cells, and in activated CD4+ memory T-lymphocytes.
Background
CD25 (IL-2Rα) is the 55 kDa subunit of the interleukin 2 receptor alpha chain which has been shown to play roles in lymphocyte differentiation, activation, and proliferation. IL-2Rα is a transmembrane glycoprotein comprised of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. IL-2Rα heterodimerizes with IL-2Rβ (CD122) and IL-2Rγ (CD132) and forms the IL-2 receptor complex. IL-2Rα by itself binds IL-2 with relatively low affinity, while the IL-2 receptor complex binds IL-2 with high affinity. IL-2Rα mutations cause severe immunodeficiency.

Antigen Details

PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Xi, X. et al. (2016) Front. Med. 10(3): 311–319
2. Hong, X. et al.(2015) Biochim Biophys Acta. 1853(2):489-99
Indirect Elisa Protocol
Flow Cytometry
IF Staining
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.