Anti-Human CD4 (Clone OKT-4) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Human CD4 (Clone OKT-4) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C502

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Clone
OKT-4
Target
CD4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
T4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4, RnasL
Isotype
IgG2b κ
Applications
IHC FF
,
IHC FFPE
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone OKT-4 has been validated for use on CODEX® using human tonsil formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue.
Tissue Screened
Tonsil
Tissue Preparation
FF (Fresh Frozen)
FFPE (Formalin-Fixed Paraffin-Embedded)

Antibody and Reporter Details

Reactivity Species
Human
Host Species
Mouse
Concentration
0.5 mg/ml
Immunogen
Human peripheral T cells
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD4 (Clone OKT-4) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2893563
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone OKT-4 recognizes Human CD4. This clone recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not interfere with each other.
Antigen Distribution
CD4 is expressed on most thymocytes, a subset of T cells, and monocytes/macrophages, and may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues.
Background
CD4 is a 55 kD single-chain type I transmembrane glycoprotein and a member of the Ig superfamily. It is involved in the recognition of MHC class II molecules. Furthermore, CD4 is the primary receptor for HIV. CD4 initiates the early phase of T-cell activation via its association with the T-cell receptor complex and protein tyrosine kinase, Lck. In addition, CD4 functions as a mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Crotty, S. et al. (2019) Cell Rep 29(7):1756-66.
2. Ploss, A. et al. (2018) Nat Commun 9(1):5031
3. Lone, YC. et al. (2017) PLoS One.12(4): e0173754.
IHC
IHC
PhenoCycler®

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.