Anti-Human CD4 (Clone OKT-4) – Purified

Anti-Human CD4 (Clone OKT-4) – Purified

Product No.: C9210

[product_table name="All Top" skus="C163"]

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Clone
OKT-4
Target
CD4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
T4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4, RNASL
Isotype
IgG2b κ
Applications
ELISA
,
FC
,
ICC
,
IF Staining
,
IHC FF
,
IP
,
N
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Human peripheral T cells
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA
FC
WB
Additional Applications Reported In Literature ?
IF Staining
IP
IHC FF
ICC
N
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone OKT4 recognizes Human CD4. This clone recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not interfere with each other.
Antigen Distribution
CD4 is expressed on most thymocytes, a subset of T cells, and monocytes/macrophages, and may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues.
Background
CD4 is a 55 kD single-chain type I transmembrane glycoprotein and a member of the Ig superfamily. It is involved in the recognition of MHC class II molecules. Furthermore, CD4 is the primary receptor for HIV. CD4 initiates the early phase of T-cell activation via its association with the T-cell receptor complex and protein tyrosine kinase, Lck. In addition, CD4 functions as a mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system.

Antigen Details

PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Crotty, S. et al. (2019) Cell Rep 29(7):1756-66.
2. Ploss, A. et al. (2018) Nat Commun 9(1):5031
3. Lone, YC. et al. (2017) PLoS One.12(4): e0173754.
Indirect Elisa Protocol
Flow Cytometry
ICC
IF Staining
IHC FF
Immunoprecipitation Protocol
N
General Western Blot Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.