Anti-Human CD4 (Clone OKT-4) – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C9200
Clone
OKT-4
Protein
CD4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD4; T4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
Isotype
Mouse
IgG2b κ
Applications
CODEX®
,
ELISA
,
FC
,
ICC
,
IF Staining
,
IHC FF
,
IHC FFPE
,
IP
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C9200- 1mg
1 mg
$98.00
In stock
Max:
Min: 1
Step: 1
C9200- 5mg
5 mg
$325.00
In stock
Max:
Min: 1
Step: 1
C9200- 25mg
25 mg
$875.00
In stock
Max:
Min: 1
Step: 1
C9200- 50mg
50 mg
$1,450.00
In stock
Max:
Min: 1
Step: 1
C9200- 100mg
100 mg
$2,125.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Human peripheral T cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA
FC
WB
Other Applications Reported In Literature ?
PhenoCycler-Fusion (CODEX)®
IF Staining
IP
IHC FF
IHC FFPE
ICC
N
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone OKT4 recognizes Human CD4. This clone recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not interfere with each other.
Antigen Distribution
CD4 is expressed on most thymocytes, a subset of T cells, and monocytes/macrophages, and may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues.
Background
CD4 is a 55 kD single-chain type I transmembrane glycoprotein and a member of the Ig superfamily. It is involved in the recognition of MHC class II molecules. Furthermore, CD4 is the primary receptor for HIV. CD4 initiates the early phase of T-cell activation via its association with the T-cell receptor complex and protein tyrosine kinase, Lck. In addition, CD4 functions as a mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Crotty, S. et al. (2019) Cell Rep 29(7):1756-66.
2. Ploss, A. et al. (2018) Nat Commun 9(1):5031
3. Lone, YC. et al. (2017) PLoS One.12(4): e0173754.
Elisa Sandwich Protocol
Flow Cytometry
IHC
IHC
Immunoprecipitation Protocol
General Western Blot Protocol