Anti-Human CD4 (Clone OKT-4) – Purified in vivo GOLD™ Functional Grade
Anti-Human CD4 (Clone OKT-4) – Purified in vivo GOLD™ Functional Grade
Product No.: C9200
Clone OKT-4 Target CD4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD4; T4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 Isotype Mouse IgG2b κ Applications ELISA , FC , ICC , IF Staining , IHC FF , IHC FFPE , in vivo , IP , N , PhenoCycler® , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen Human peripheral T cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829879 Applications and Recommended Usage? Quality Tested by Leinco ELISA FC WB Additional Applications Reported In Literature ? PhenoCycler-Fusion (CODEX)® IF Staining IP IHC FF IHC FFPE ICC N Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone OKT4 recognizes Human CD4. This clone recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not interfere with each other. Background CD4 is a 55 kD single-chain type I transmembrane glycoprotein and a member of the Ig superfamily. It is involved in the recognition of MHC class II molecules. Furthermore, CD4 is the primary receptor for HIV. CD4 initiates the early phase of T-cell activation via its association with the T-cell receptor complex and protein tyrosine kinase, Lck. In addition, CD4 functions as a mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Antigen Distribution CD4 is expressed on most thymocytes, a subset of T cells, and monocytes/macrophages, and may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone OKT-4 is a mouse monoclonal antibody that specifically recognizes human CD4, not mouse CD4. Its in vivo applications in mice are limited to situations where human CD4 is expressed, such as in mouse models reconstituted with human immune systems (humanized mice). Common in vivo applications of clone OKT-4 in mice:
Important context and limitations:
Summary table:
Conclusion: The most commonly used antibodies or proteins studied alongside OKT-4 (an anti-human CD4 monoclonal antibody) in the literature typically target other immune cell markers or CD4 epitopes, depending on the research context. Key antibodies or proteins frequently used with OKT-4 include:
Summary Table: Commonly Used Antibodies/Proteins with OKT-4
When cited in immunology and HIV research, OKT-4 is specifically associated with human CD4+ T cell detection and functional assays, and often combined with these markers to give a comprehensive view of immune cell populations and their responses. Key Findings for Clone OKT-4 in Scientific LiteratureClone OKT-4 refers to a well-characterized monoclonal antibody against the human CD4 molecule, which marks helper T cells and some cytotoxic T cells (CTLs). The scientific citations reveal two primary research contexts for OKT-4: immunology (particularly T cell function and specificity) and clinical/diagnostic applications. Below are the key findings from relevant literature. Immunological Role and Specificity
Clinical and Molecular Applications
Summary Table: Properties of OKT-4+ (CD4+) T Cell Clones
ConclusionClone OKT-4 and the OKT4 antibody have been pivotal in demonstrating that CD4+ T cells are not exclusively helper cells but can also exhibit cytotoxic activity against MHC class II-expressing targets. The antibody is widely used for phenotyping, isolating CD4+ T cells, and studying their functional diversity. Its specific binding characteristics (e.g., D3 domain, non-interference with HIV binding) make it valuable for both basic and translational immunology research. Dosing regimens for OKT-4 (anti-CD4, clone OKT4) vary considerably based on the specific mouse model, disease context, and study objectives, though comprehensive standardized protocols remain limited in the literature. The available evidence indicates that researchers must often adapt dosing strategies based on their particular experimental requirements. General Dosing ConsiderationsThe variability in OKT-4 dosing reflects the broader challenges in antibody administration across different mouse models. Factors influencing dosing decisions include the administration route, the specific disease model being studied, and whether the goal is depletion, modulation, or blockade of CD4+ cells. Because OKT-4 targets human CD4, its use is typically restricted to humanized mouse models rather than conventional mice. Documented Dosing ExamplesIn humanized mouse models, specific dosing regimens have been documented with considerable range. For instance, in studies using HuCD4/Tg mice, researchers have employed three daily doses at either 1 mg/kg or 100 mg/kg to achieve cumulative doses of 3 mg/kg and 300 mg/kg respectively. This represents a 100-fold difference in dosing, highlighting the substantial variation in experimental approaches. Broader Context of Antibody Dosing in Mouse ModelsThe challenge of establishing optimal dosing regimens for therapeutic antibodies in mice is well-recognized across immunology research. Dosing frequencies in preclinical mouse studies typically differ from human protocols, with mice often receiving more frequent administrations. Researchers commonly aggregate doses over standardized time windows to facilitate comparison—for example, converting multiple doses given every few days into an equivalent single dose or weekly dose for analysis purposes. The lack of standardized protocols for OKT-4 dosing necessitates that researchers carefully consider their experimental endpoints and conduct pilot studies to determine appropriate doses for their specific applications. References & Citations1. Crotty, S. et al. (2019) Cell Rep 29(7):1756-66. 2. Ploss, A. et al. (2018) Nat Commun 9(1):5031 3. Lone, YC. et al. (2017) PLoS One.12(4): e0173754. Technical ProtocolsCertificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
