Anti-Human CD57 [Clone VC1.1] — Alexa Fluor® 488

Purified Recombinant Human CD57 (>98%)

This Alexa Fluor® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Alexa Fluor® is a trademark of Life Technologies Corporation.

Liquid

This Alexa Fluor® 488 conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2-8°C Wet Ice

Blue Laser (488 nm) Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Mouse Anti-Human CD57 (Clone VC1.1) recognizes an epitope on Human CD57. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Anti-CD57 may be used in flow cytometry, Western blotting and immunohistology on formalin fixed paraffin embedded tissue sections. The antibody is useful for enumeration of NK subsets and T-lymphocyte subsets, studies of neural tissue, malignant tissues and AIDS-related complex.

The CD57 antigen is present on 15-20% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of T-lymphocytes. This carbohydrate is also present on N-CAM in the nervous system.

Binds to L-selectin and P-selectin in a calcium-dependent manner, also binds to second globular domain of E8 laminin fragment.

1. Barnstable, C. et al. (1995) Brain Research 559:118 2. Leukocyte Typing V (1995) Oxford University Press: Code No. NK67 3. Arimatsu, Y. et al. (1987) J. Neuroscience 7:1250 4. Abo, T., and Balch, C.M. et al. (1981) J. Immunol. 127:1024