Anti-Human CXCL10 (Clone 33036)

Pricing & Details

Product No.C1389
Clone
33036
Protein
CXCL10
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IP-10, SCYB10, Crg-2
Isotype
Mouse
IgG1
Applications
CyTOF®
,
ELISA Cap
,
FC
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1389-25 µg
25 µg
$195.00
In stock
Max:
Min: 1
Step: 1
C1389-100 µg
100 µg
$369.00
In stock
Max:
Min: 1
Step: 1
C1389-500 µg
500 µg
$799.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human CXCL10 (Accession # P02778.2)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is PN:I-275 at a concentration of approximately 0.1-0.4 µg/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 2 ng/well. A suggested standard for this assay is PN:I-202.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCL10. A suggested method would be to stain cells expressing CXCL10 with 0.25 µg per 1-5 x 106 cells in a total staining volume of ≤ 200 µl followed by PN:M1189.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human CXCL10 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CXCL10 (at 0.2 ng/ml) is 0.5 - 2 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CXCL10 (Clone 33036) recognizes an epitope on Human CXCL10. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family. CXCL10 is secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells and fibroblasts.1 CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.2,3 CXCL10 plays a role in the regulation of tubular cell proliferation following renal ischemia-reperfusion injury.4

Antigen Details

Protein
PubMed

References & Citations

1. Luster, AD. et al.(1985) Nature 315: 672
2. Dufour, JH. et al.(2002) J. Immunol. 168
3. Angiolillo, AL. et al.(1995) J. Exp. Med. 182: 155
4. Kaneko, S. et al.(2008) Nephron Exp. Nephrol. 109:(1):c29-38
Elisa Sandwich Protocol
Flow Cytometry

Formats Available

Prod No.
Description
C1389
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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