Anti-Mouse CXCL10 (Clone 134013)

Pricing & Details

Product No.C1423
Clone
134013
Protein
CXCL10
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Chemokine (C-X-C Motif) Ligand 10, C7, IFI10, INP10, IP-10, SCYB10, Crg-2, GIP-10, Mob-1
Isotype
Rat
IgG2a
Applications
ELISA Cap
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1423-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
C1423-100 µg
100 µg
$399.00
In stock
Max:
Min: 1
Step: 1
C1423-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
E. coli-Derived Recombinant Mouse CXCL10
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-276 at a concentration of approximately 2 µg/ml. A suggested standard for this assay is PN:I-229.
Western Blotting: To detect Mouse CXCL10 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL10 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse CXCL10 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CXCL10 (at 0.5 ng/ml) is 10-40 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Chemokine (C-X-C Motif) Ligand 10 (CXCL10) (Clone 134013) recognizes an epitope on Mouse CXCL10. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family. CXCL10 is secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells and fibroblasts.1 CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.2,3 CXCL10 plays a role in the regulation of tubular cell proliferation following renal ischemia-reperfusion injury.4

Antigen Details

Protein
PubMed

References & Citations

1. Luster, AD. et al.(1985) Nature 315: 672
2. Dufour, JH. et al.(2002) J. Immunol. 168
3. Angiolillo, AL. et al.(1995) J. Exp. Med. 182: 155
4. Kaneko, S. et al.(2008) Nephron Exp. Nephrol. 109:(1):c29-38
Elisa Sandwich Protocol
General Western Blot Protocol

Formats Available

Prod No.
Description
C1423
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Leinco Technologies uses cookies to improve your experience and our website service. By continuing to browse our website, you accept our cookie policy. Ok