Anti-Mouse CXCL10 (Clone 134013)
Anti-Mouse CXCL10 (Clone 134013)
Product No.: C1423
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Clone 134013 Target CXCL10 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Chemokine (C-X-C Motif) Ligand 10, C7, IFI10, INP10, IP-10, SCYB10, Crg-2, GIP-10, Mob-1 Isotype IgG2a Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen E. coli-Derived Recombinant Mouse CXCL10 Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828483 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-276 at a concentration of approximately 2 µg/ml. A suggested standard for this assay is PN:I-229. Western Blotting: To detect Mouse CXCL10 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL10 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse CXCL10 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CXCL10 (at 0.5 ng/ml) is 10-40 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse Chemokine (C-X-C Motif) Ligand 10 (CXCL10) (Clone 134013) recognizes an epitope on Mouse CXCL10. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family. CXCL10 is secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells and fibroblasts.1 CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.2,3 CXCL10 plays a role in the regulation of tubular cell proliferation following renal ischemia-reperfusion injury.4 Antigen DetailsPubMed References & Citations1. Luster, AD. et al.(1985) Nature 315: 672 2. Dufour, JH. et al.(2002) J. Immunol. 168 3. Angiolillo, AL. et al.(1995) J. Exp. Med. 182: 155 4. Kaneko, S. et al.(2008) Nephron Exp. Nephrol. 109:(1):c29-38 Technical Protocols N  |
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I-276 |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
