Anti-Human CXCL9 (Clone 49106)

Pricing & Details

Product No.M1103
Clone
49106
Protein
CXCL9
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Chemokine (C-X-C Motif) Ligand 9, CMK, Humig, MIG, SCYB9, Crg-10
Isotype
Mouse
IgG1
Applications
CyTOF®
,
ELISA Cap
,
FC
,
ICC
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
M1103-25 µg
25 µg
$195.00
In stock
Max:
Min: 1
Step: 1
M1103-100 µg
100 µg
$440.00
In stock
Max:
Min: 1
Step: 1
M1103-500 µg
500 µg
$935.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human CXCL9 (Accession # Q07325)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 - 8 µg/ml. A suitable detection antibody is PN:M131 at a concentration of approximately 0.1 - 0.4 µg/ml. A suggested standard for this assay is PN:M160.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MIG. A suggested method would be to stain cells expressing MIG with 0.25 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤ 200 µl followed by PN:M1189.
Western Blotting: To detect Human CXCL9 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL9 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M160.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human MIG bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MIG (at 1 ng/ml) is 15 - 30 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Monokine Induced by Interferon-Gamma (MIG) (Clone 49106) recognizes an epitope on Human MIG. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. Mig may be important not only to recruit T cells to peripheral inflammatory sites, but also in some cases to maximize interactions among activated T cells, B cells, and dendritic cells within lymphoid organs to provide optimal humoral responses to pathogens.1

Antigen Details

Protein
PubMed

References & Citations

1. Farber, JM. et al. (2002) J. Immunol. 169: 1433
Elisa Sandwich Protocol
Flow Cytometry
General Western Blot Protocol

Formats Available

Prod No.
Description
M1103
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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