Purified Recombinant Human CXCL9 (Accession # Q07325)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 - 8 µg/ml. A suitable detection antibody is PN:M131 at a concentration of approximately 0.1 - 0.4 µg/ml. A suggested standard for this assay is PN:M160.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MIG. A suggested method would be to stain cells expressing MIG with 0.25 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤ 200 µl followed by PN:M1189.
Western Blotting: To detect Human CXCL9 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL9 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M160.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human MIG bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MIG (at 1 ng/ml) is 15 - 30 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Monokine Induced by Interferon-Gamma (MIG) (Clone 49106) recognizes an epitope on Human MIG. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. Mig may be important not only to recruit T cells to peripheral inflammatory sites, but also in some cases to maximize interactions among activated T cells, B cells, and dendritic cells within lymphoid organs to provide optimal humoral responses to pathogens.1
References & Citations
1. Farber, JM. et al. (2002) J. Immunol. 169: 1433
Products are for research use only. Not for use in diagnostic or therapeutic procedures.