Anti-Human ICAM-1 – Purified in vivo PLATINUM™ Functional Grade

Clone 15.2 is a monoclonal antibody primarily targeting ICAM-1 (CD54) and is widely used in in vivo mouse studies to investigate immune responses, inflammation, and cell adhesion processes. It is applied for blocking or detecting ICAM-1 on murine tissues and cells, with research focusing on its role in leukocyte trafficking, endothelial activation, and disease models involving immune cell extravasation.

Key usage contexts in in vivo mouse studies:

• Blocking ICAM-1 function: Clone 15.2 is administered to mice to block ICAM-1-mediated interactions, which can inhibit leukocyte endothelial adhesion and transmigration, relevant in models of inflammation and autoimmune disease.
• Functional studies: Used to interrogate the role of ICAM-1 in immune cell recruitment and tissue infiltration by preventing its engagement with LFA-1 on leukocytes.
• Detection and characterization: Employed in immunohistochemistry (IHC), flow cytometry, and immunoprecipitation to characterize ICAM-1 expression in mouse tissues, including during pathological conditions or immune activation.

Additional details:

• Antibody features: Mouse IgG1 isotype, reactivity with mouse and human ICAM-1, available in low-endotoxin formulations designed for in vivo applications to minimize inflammatory artifacts.
• Administration: Delivered via intravenous or intraperitoneal injection, typically in disease models such as EAE (experimental autoimmune encephalomyelitis), ischemia-reperfusion injury, or tumor biology to modulate immune cell behavior.
• Mechanistic studies: Used to demonstrate ICAM-1’s necessity for leukocyte trafficking and to test therapeutic strategies targeting adhesion molecules.

If your query was about clone M5/114.15.2, which targets mouse MHC class II for blocking antigen-presenting functions or characterizing immune populations in in vivo mouse models, please specify, as this is a distinct clone from anti-ICAM-1 15.2.

In summary, clone 15.2 (anti-ICAM-1) is most commonly used for in vivo inhibition and detection of ICAM-1 in mouse models, enabling study of immune cell adhesion, migration, and associated inflammatory processes.

Storage Temperature for Sterile Packaged Clone 15.2

The appropriate storage temperature for a sterile packaged Clone 15.2—a monoclonal antibody or similar biological reagent—depends on several factors, including the nature of the clone, the manufacturer’s recommendations, and the intended storage duration. However, based on general best practices for monoclonal antibodies and sterile biological products, the following guidelines apply:

Recommended Temperature Ranges

• Short-term Use (Days to Weeks): Most monoclonal antibodies, including clone 15.2, are best stored between 2°C and 8°C (refrigerated conditions) to maintain stability and avoid repeated freeze-thaw cycles that can degrade the antibody.
• Long-term Storage (Months to Years): For extended storage, ?20°C is commonly recommended. Antibodies stored at this temperature can remain stable for years without significant loss of activity. For even longer stability (especially beyond a year), ?80°C is advised, as this further minimizes degradation and microbial growth.
• Avoid Room Temperature: Storage at room temperature is generally not recommended for extended periods, as this can lead to rapid loss of antibody activity.

Additional Storage Considerations

• Aliquoting: To prevent repeated freeze-thaw cycles, divide the antibody into small aliquots and freeze only what you need for each experiment.
• Avoid Frost-Free Freezers: These freezers undergo temperature fluctuations, which can harm antibody stability.
• Sterility and Packaging: For sterile medical devices and reagents, the storage area should be dry, clean, protected from light and dust, and maintained at a maximum of 25°C if not refrigerated or frozen. However, for active biological reagents like antibodies, refrigeration or freezing is strongly preferred.

Default Recommendation

Unless the manufacturer of Clone 15.2 specifies otherwise, store at 2–8°C for short-term use and at ?20°C to ?80°C for long-term storage. Always consult the product datasheet or manufacturer for clone-specific instructions, as stability can vary.

Summary Table

Always confirm with the manufacturer’s datasheet for clone 15.2, as specific recommendations may vary.

In the literature, CD54 (ICAM-1) targeted by the 15.2 antibody is often used in various research applications. While specific combinations of other antibodies or proteins used directly with the 15.2 antibody may not be extensively detailed in the provided search results, here are some commonly used antibodies or detection methods in the broader context:

1. Protein-A/G: This protein is often used for detecting antibodies across different species, including in assays related to biotherapeutic drugs, making it a versatile tool in immunological research.

2. Secondary Antibodies like Anti-Mouse IgG: These are commonly used in conjunction with primary antibodies like 15.2 (which is a mouse IgG1 antibody) to enhance detection in techniques such as western blot, immunoprecipitation, and immunohistochemistry.

3. Detection Labels (e.g., Horseradish Peroxidase, Alkaline Phosphatase): These enzymes are frequently used in conjunction with secondary antibodies to facilitate detection through colorimetric or chemiluminescent signals.

4. SULFO-TAG Labeled Conjugates: These are used in electrochemiluminescence assays for detecting antibodies, providing high sensitivity and are particularly useful in clinical settings for ADA (anti-drug antibody) testing.

For specific applications involving ICAM-1 (CD54) targeted by the 15.2 antibody, these antibodies are often used in studies related to immune cell adhesion, inflammation, and viral infection, especially in the context of rhinovirus entry into host cells. However, direct combinations of these antibodies with 15.2 are not explicitly outlined in the search results.

I'm sorry, but none of the search results provide direct information about the key findings from specific scientific studies that cite or utilize "clone 15.2" (such as the anti-ICAM1 antibody clone 15.2 or a similar immunological reagent). Most references to clone 15.2 in the search results relate only to the product description of antibodies—specifically, their reactivity with ICAM-1 (CD54), species specificity (human or pig), and recommended applications (e.g., quantitative analysis, flow cytometry). There are no detailed scientific results or peer-reviewed findings cited here for experiments performed with clone 15.2.

Summary of Available Information

• Clone 15.2 Antibody Properties:
  Clone 15.2 is described as a mouse monoclonal antibody specific for ICAM-1 (CD54), a glycoprotein involved in leukocyte-endothelial interactions. One version targets human ICAM-1, while another is also reactive with pig ICAM-1. Both are suitable for quantitative and flow cytometry applications, but the search results do not describe what experimental findings or insights were obtained using this clone.
• Lack of Scientific Findings:
  The search results do not include any excerpts, summaries, or citations of research papers that report experimental results (e.g., functional assay outcomes, disease mechanisms, or immunological discoveries) using clone 15.2. Without access to the primary scientific literature, I cannot provide a summary of such findings.
• Other "15.2" Mentions:
  Other search results mentioning "15.2" refer to unrelated topics, such as citation patterns, retraction analysis, fMRI studies, or different antibody clones (e.g., M5/114.15.2), which are not relevant to the immunology of ICAM-1 or clone 15.2.

Conclusion

While clone 15.2 anti-ICAM1 monoclonal antibodies are commercially available and have well-defined targets and intended uses, the search results do not describe any key scientific findings from research employing this clone. For a thorough understanding of the experimental outcomes and citations involving clone 15.2, direct consultation of peer-reviewed articles where this clone was used would be necessary—information not present in the current search results.