Purified Recombinant Human IgG Fc Specific (>98%)
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze.
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%
There is no detectable binding to bovine, goat, horse sheep IgG.
NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HP6043) when conjugated to HRPO is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific (Part No.: I-1200)
Country of Origin
Applications and Recommended Usage?
Quality Tested by Leinco
FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Red Laser (652 nm)
Mouse Anti-Human IgG Fc Specific (Clone HP6043) recognizes an epitope on Human IgG Fc Specific. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Surface IgG is expressed on approximately 2% of normal human peripheral blood cells.
References & Citations
1. Reimer, C. B. et al. (1984) Hybridoma 3:263 2. Jefferies, R. et al. (1985) Immunol. Letters 10:223 3. Phillips, D. J. et al. (1987) Immunol. Letters 17:159
Products are for research use only. Not for use in diagnostic or therapeutic procedures.