Anti-Human IL-11

Purified Recombinant Human IL-11 (>98%)

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

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Neutralization: Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. Nordan, R. P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND₅₀) is typically 0.06-0.48 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑11.

Rabbit Anti-Human Interleukin-11 (IL-11) recognizes Human IL-11. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Interleukin 11 (IL-11) is a multifunctional cytokine isolated from bone marrow-derived stromal cells. It is a key regulator of multiple events in hemtaopoiesis, most notably the stimulation of megakaryocyte maturation.¹ It is also known under the names Adipogenesis inhibitory factor (AGIF)² and Oprelvekin. IL-11 improves platelet recovery after chemotherapy-induced thrombocytopenia, induces acute phase proteins, modulates antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. Besides from lymphopoietic/hematopoietic and osteotrophic properties, it has functions in many tissues such as brain, gut and testis.

1. Williams, DA. et al. (1990) Proc. Natl. Acad. Sci. USA87: 7512
2. Takiguchi, Y. et al. (1991) FEBS Lett. 283: 199