Anti-Human Pro/Active MMP-8

Product No.: M1290


Clone 100619 Target MMP-8 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CLG1, HNC, PMNL-CL Isotype IgG2b Applications ELISA Cap , IP , WB


Select Product Size 25 µg — $250.00 100 µg — $500.00 500 µg — $0	Qty Max: Min: 1 Step: 1 Select A Size ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human MMP-8 (Accession # AAZ38714) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831285 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is PN:M1263 at a concentration of approximately 50 ng/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 2 ng/well. A suggested standard for this assay is PN:M1252. Western Blotting: To detect Human MMP-8 this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MMP-8 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M1252. Additional Applications Reported In Literature ? Immunoprecipitation: This antibody can be coupled to sepharose beads and used to immunoprecipitate human MMP-8 from conditioned cell culture medium at a concentration of 25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Matrix Metalloproteinase 8 (MMP-8) (Clone 100619) recognizes an epitope on Human MMP-8. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Matrix metallopeptidase 8 (neutrophil collagenase), also known as MMP8, is a part of the MMP family which are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by MMP8 is stored in secondary granules within neutrophils and is activated by autolytic cleavage. MMP8 is a neutral metalloproteinase of the fibrillar collagenase family that also includes MMP-1 and MMP-13. In contrast to the other collagenases, MMP-8 has a very limited tissue distribution, thought to be restricted to neutrophils and chondrocytes.¹ Its function is degradation of type I, II and III collagens. MMP-8 is the predominant collagenase present in normal healing wounds and suggests that overexpression and activation of this collagenase may be involved in the pathogenesis of nonhealing chronic ulcers.² MMP-8 might also be useful in monitoring periodontal disease in diabetes.³ PubMed MMP-8 NCBI Gene Bank ID 4317 UniProt.org Information on Uniprot.org References & Citations 1. Klebe, RJ. et al. (2001) Matrix Biol. 20: 577 2. Yager, DR. et al. (1999) J Surg Res. 81: 189 3. Uusitupa, M. et al. (2000) J Periodontal Res. 35: 259 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human MMP-8 (Accession # AAZ38714)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831285

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is PN:M1263 at a concentration of approximately 50 ng/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 2 ng/well. A suggested standard for this assay is PN:M1252.
Western Blotting: To detect Human MMP-8 this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MMP-8 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M1252.

Additional Applications Reported In Literature ?

Immunoprecipitation: This antibody can be coupled to sepharose beads and used to immunoprecipitate human MMP-8 from conditioned cell culture medium at a concentration of 25 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Matrix Metalloproteinase 8 (MMP-8) (Clone 100619) recognizes an epitope on Human MMP-8. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Matrix metallopeptidase 8 (neutrophil collagenase), also known as MMP8, is a part of the MMP family which are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by MMP8 is stored in secondary granules within neutrophils and is activated by autolytic cleavage. MMP8 is a neutral metalloproteinase of the fibrillar collagenase family that also includes MMP-1 and MMP-13. In contrast to the other collagenases, MMP-8 has a very limited tissue distribution, thought to be restricted to neutrophils and chondrocytes.¹ Its function is degradation of type I, II and III collagens. MMP-8 is the predominant collagenase present in normal healing wounds and suggests that overexpression and activation of this collagenase may be involved in the pathogenesis of nonhealing chronic ulcers.² MMP-8 might also be useful in monitoring periodontal disease in diabetes.³

PubMed

MMP-8

NCBI Gene Bank ID

4317

UniProt.org

Information on Uniprot.org

References & Citations

1. Klebe, RJ. et al. (2001) Matrix Biol. 20: 577
2. Yager, DR. et al. (1999) J Surg Res. 81: 189
3. Uusitupa, M. et al. (2000) J Periodontal Res. 35: 259

View product citations for antibody PRODUCT_CODE on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
M1290	Anti-Human Pro/Active MMP-8
M1301	Anti-Human MMP-8
M1263	Anti-Human MMP-8 – Biotin

Formats Available

Prod No.	Description
M1290	Anti-Human Pro/Active MMP-8

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

NEW Products!

Anti-Human Pro/Active MMP-8