Anti-Human Prolactin [Clone 174] — Purified

Anti-Human Prolactin [Clone 174] — Purified

Product No.: P101

[product_table name="All Top" skus="P101"]

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Clone
174
Target
Prolactin
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Mammotropin, Luterotropic Hormone (LTH), Lutetropin
Isotype
IgG

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Data

This graph shows that the Leinco antibody pair P100 (gold conjugate) and P101 (nitrocellulose membrane) exhibits a strong, curvilinear dose-response for prolactin. The data indicates high analytical sensitivity, with an estimated Limit of Detection (LoD) of ≤0.625 ng/mL.Prolactin LOD Graph
The gold conjugate (CG) antibody (Leinco SKU P100) paired with the nitrocellulose membrane (NCM) antibody (Leinco SKU P101) demonstrates a strong, curvilinear dose-response across the tested concentration range. The data supports an excellent analytical sensitivity, with an estimated Limit of Detection (LoD) of ≤0.625 ng/mL from prolactin samples.
A series of seven lateral flow assay (LFA) strips showing the detection of prolactin. The test line is not visible on the first strip, but becomes progressively darker on the remaining strips as the concentration of prolactin increases. The control line remains consistently strong across all concentrations.Prolactin LFA Image
The prolactin Lateral Flow Assay image demonstrates visible detection at the test line starting at the 2.5 ng/mL concentration, with the signal intensity clearly increasing up to 25 ng/mL, while the control line remains robustly positive across all tested concentrations.
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human Prolactin (>98%)
Product Concentration
≥ 5.0 mg/ml
Purity
≥95% by SDS Page
Formulation
This purified antibody is supplied in 0.05 M phosphate buffered saline (PBS), pH 7.2 - 7.4, containing 0.1% sodium azide. as a preservative.
State of Matter
Liquid
Storage and Handling
This Purified Antibody is stable when stored at 2-8°C. Do not freeze.
Cross Reactivity
Paired with Leinco Prod. No. P100, Clone No. 172: Pro 100%, BhCG < 1%, ihCG < 1%, LH <1%, FSH < 1%. Paired with Leinco Prod. No. P102, Clone No. 180: Pro 100%, BhCG < 1%, ihCG < 1%, LH <1%, FSH < 1%.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Anti-Human Prolactin (Clone 174) recognizes Human Prolactin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Prolactin (PRL) or Luteotropic hormone (LTH) is best known as the pituitary modulator of lactation and reproduction.1 Prolactin is a multifaceted hormone that is capable of modulating hundreds of physiological processes in adult vertebrates.2 PRL promotes proliferation, survival and migration of cancer cells acting via the prolactin receptor (PRLR).3 It also modulates maternal behavior and mediates hypothalamic pituitary adrenal axis inhibition during lactation via PRL receptors in the brain.4 Prolactin also has a number of other effects including contributing to surfactant synthesis of the fetal lungs at the end of the pregnancy and immune tolerance of the fetus by the maternal organism during pregnancy. It also decreases normal levels of sex hormones — estrogen in women and testosterone in men.5 Prolactin is a single chain polypeptide of 199 amino acids with a molecular weight of about 24,000 daltons. Its structure is similar to that of growth hormone and placental lactogen. The molecule is folded due to the activity of three disulfide bonds. Significant heterogeneity of the molecule has been described, thus bioassays and immunoassays can give different results due to differing glycosylation, phosphorylation, sulfation, as well as degradation. The non-glycosylated form of prolactin is the dominant form of prolactin that is secreted by the pituitary gland. Increased serum concentrations of prolactin during pregnancy cause enlargement of the mammary glands of the breasts and increases the production of milk. However, the high levels of progesterone during pregnancy act directly on the breasts to stop ejection of milk. It is only when the levels of this hormone fall after childbirth that milk ejection is possible.
PubMed
Research Area
Hormones

References & Citations

1. Paus, R. et al. (2012) Arch Dermatol Res. 304(2):115-8.
2. Zhu, Y. et al. (2008) Comp Biochem Physiol C Toxicol Pharmacol. 148(4):370-80.
3. Panina, S. et al. (2009) J Endocrinol. 201(1):115-28.
4. Neuwmann, ID. et al. (2009) Endocrinology. 150(4):1841-9.
5. Molitch MD., ME. (2005) Mayo Clinic Proceedings 80(8):1050-1057
6. Gout, PW. et al. (1980) Cancer Research 40:2433-36.
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Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.