Anti-Human TRAIL R3 (Clone 90905)
Anti-Human TRAIL R3 (Clone 90905)
Product No.: T450
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Clone 90905 Target TRAIL R3 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TRID, TNFRSF10C, CD263, DCR1, LIT, MGC149501, MGC149502, TNF-Related Apoptosis-Inducing Ligand Receptor 3 Isotype IgG1 Applications ELISA Cap , IHC FFPE , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen NS0-Derived Recombinant Human TRAIL Receptor 3 Extracellular Domain Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831950 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human TRAIL R3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TRAIL R3 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. ELISA Capture: Suitable for use at concentration of 2-8 µg/mL. Use in combination with: Detection Reagent: Human TRAIL R3/TNFRSF10C Biotinylated Antibody. Standard: Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera Protein. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human TRAIL R3 (Clone 90905) recognizes an epitope on Human TRAIL R3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with rhTRAIL R1, rhTRAIL R2, or rhTRAIL R4. Background TNF-related apoptosis-inducing ligand receptor 3 (TRAIL R3) is an antagonist decoy receptor1 that can inhibit TRAIL-induced apoptosis.2 TRAIL R3 is a plasma membrane-bound protein capable of high affinity interaction with the TRAIL ligand.3 Antigen DetailsPubMed References & Citations1. Fornace Jr, AJ. et al. (1999) Oncogene 18: 4153 2. Büchler, MW. et al. (2001) Anticancer Res. 21: 3153 3. Smith, CA. et al. (1997) J Exp Med. 186: 1165 Technical Protocols IHC FFPE  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
