Anti-Influenza B Neuraminidase [Clone B3]

Influenza B Lee/40

PBS, pH 7.4.

Liquid

Purified by Protein G affinity chromatography

This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Next Day 2-8°C

Mouse Monoclonal Antibody specific to Influenza B Neuraminidase

Mechanism of Action: Influenza Neuraminidase Neuraminidases are critical surface enzymes responsible for cleaving sialic acid groups from glycoproteins. In the context of the Influenza B virus, viral neuraminidase (NA) plays a pivotal role in the lifecycle of the pathogen. Structurally, Influenza neuraminidase is composed of four identical subunits arranged in a square tetramer, anchored to the virus surface by a long protein stalk. The enzyme’s active sites are located in deep depressions on the upper surface, where they bind to polysaccharide chains and clip off terminal sugars.

Neuraminidase vs. Hemagglutinin in Viral Infection
-Neuraminidase (NA) and Hemagglutinin (HA) are the two major membrane glycoproteins found on the surface of the influenza virus. They function in concert but with opposing roles:

- Hemagglutinin (HA): Initiates infection by binding to sialic acid-containing receptors on the surface of host cells.

- Neuraminidase (NA): Facilitates viral release. It acts as a receptor-destroying enzyme by cleaving the HA-sialic acid bond between newly formed virions and host cell receptors during budding.

Research Applications for Anti-Influenza B Neuraminidase Antibodies
Because of its essential role in viral spread, neuraminidase is a primary target for anti-influenza virus therapy development (such as neuraminidase inhibitors) and vaccine research. High-quality Influenza antibodies are indispensable tools for these studies.

This Anti-Influenza B Neuraminidase Antibody (Cat. No. 19601) is specifically designed to aid researchers in:
- Influenza Diagnosis: Detecting the presence of the Influenza B virus in biological samples.

- Vaccine Development: Characterizing viral strains and assessing the potency of neuraminidase antigens.

-Therapeutic Research: Screening and validating potential antiviral compounds that inhibit neuraminidase activity.

Influenza B Neuraminidase (NA) is a receptor-destroying enzyme essential for the viral life cycle. It functions as a sialidase, cleaving terminal sialic acid residues from glycoproteins on the host cell surface and on newly formed virions. This enzymatic activity prevents the aggregation of progeny viruses and detaches them from the host cell receptors (hemagglutinin-sialic acid tethering), thereby facilitating the efficient release and spread of the virus to uninfected cells.

1.) G.M. Air et al. (1990) Virology 177: 578. (Catalog no. 19601 = MAb B3, 19602 = MAb B14, 19603 = MAb B19).
2.) Matrosovich MN, Matrosovich TY, Gray T, Roberts NA, Klenk HD. Neuraminidase is important for the initiation of influenza virus infection in human airway epithelium. J Virol. 2004 Nov;78(22):12665-7. doi: 10.1128/JVI.78.22.12665-12667.2004. PMID: 15507653; PMCID: PMC525087.
3.)Creytens S, Pascha MN, Ballegeer M, Saelens X, de Haan CAM. Influenza Neuraminidase Characteristics and Potential as a Vaccine Target. Front Immunol. 2021 Nov 16;12:786617. doi: 10.3389/fimmu.2021.786617. PMID: 34868073; PMCID: PMC8635103.