Anti-Mouse CD137 (4-1BB) [Clone 3H3] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD137 (4-1BB) [Clone 3H3] — Purified in vivo GOLD™ Functional Grade
Product No.: C2830
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Clone 3H3 Target 4-1BB Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TNFRSF9, 4-1BB, CD137 Antigen, T-Cell Antigen, ILA, CD137 Isotype Rat IgG2a κ Applications ELISA , FA , in vivo , WB |
Data
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Recombinant Mouse CD137 human Fc fusion protein Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737470 Applications and Recommended Usage? Quality Tested by Leinco WB ELISA Additional Applications Reported In Literature ? in vivo 4-1BB stimulation in vitro 4-1BB stimulation Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity 4-1BB antibody (clone 3H3) recognizes an epitope on mouse 4-1BB. Background 4-1BB (CD137) is a 39 kD transmembrane protein that is a member of the tumor necrosis factor (TNF) receptor family and is a co-stimulatory molecule that plays a role in T-cell-mediated proliferative response. When binding its ligand, CD137 provides costimulatory signals to both CD4 and CD8 T cells via the activation of NF-B, c-Jun and p38 downstream pathways. Crosslinking of CD137 boosts T cell proliferation, IL-2 secretion, survival and cytolytic activity. Furthermore, it can increase immune activity to eliminate tumors in mice. Agonistic 4-1BB antibodies have been reported to stimulate a more intense immune system attack on cancers. Antigen Distribution 4-1BB is expressed on activated B cells and T cells, macrophages, and dendritic cells. Ligand/Receptor 4-1BB (CDw137) PubMed NCBI Gene Bank ID UniProt.org Research Area Costimulatory Molecules . Immunology References & Citations1. Shan B et al. (2008) Cell Mol Immunol. 5: 379 2. Kwan BS et al. (1996) Cell Immunol. 169: 91 3. Kwan BS et al. (1998) Eur J Immunol. 28: 881 4. Kwan BS et al. (2009) J Immunol. 182: 4107 Technical Protocols FA   Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
