Anti-Mouse CD137 (4-1BB) Antibody – Purified in vivo GOLD™ Functional Grade

Anti-Mouse CD137 (4-1BB) Antibody – Purified in vivo GOLD™ Functional Grade

Product No.: C2830

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Clone
3H3
Target
4-1BB
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TNFRSF9, 4-1BB, CD137 Antigen, T-Cell Antigen, ILA, CD137
Isotype
IgG2a κ
Applications
ELISA
,
FA
,
in vivo
,
WB

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Product Size

Data

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Recombinant Mouse CD137 human Fc fusion protein
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
WB
ELISA
Additional Applications Reported In Literature ?
in vivo 4-1BB stimulation
in vitro 4-1BB stimulation
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
4-1BB antibody (clone 3H3) recognizes an epitope on mouse 4-1BB.
Antigen Distribution
4-1BB is expressed on activated B cells and T cells, macrophages, and dendritic cells.
Background
4-1BB (CD137) is a 39 kD transmembrane protein that is a member of the tumor necrosis factor (TNF) receptor family and is a co-stimulatory molecule that plays a role in T-cell-mediated proliferative response. When binding its ligand, CD137 provides costimulatory signals to both CD4 and CD8 T cells via the activation of NF-B, c-Jun and p38 downstream pathways. Crosslinking of CD137 boosts T cell proliferation, IL-2 secretion, survival and cytolytic activity. Furthermore, it can increase immune activity to eliminate tumors in mice. Agonistic 4-1BB antibodies have been reported to stimulate a more intense immune system attack on cancers.

Antigen Details

Ligand/Receptor
4-1BB (CDw137)
PubMed
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
.
Immunology

References & Citations

1. Shan B et al. (2008) Cell Mol Immunol. 5: 379
2. Kwan BS et al. (1996) Cell Immunol. 169: 91
3. Kwan BS et al. (1998) Eur J Immunol. 28: 881
4. Kwan BS et al. (2009) J Immunol. 182: 4107
Indirect Elisa Protocol
FA
in vivo Protocol
General Western Blot Protocol
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.