Anti-Mouse CD86

Pricing & Details

Product No.B585
Clone
GL1
Protein
CD86
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
B7-2, B70, Ly-58, CD-86
Isotype
Rat
IgG2a
Applications
CyTOF®
,
ELISA Indirect
,
FC
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
B585-25 µg
25 µg
$175.00
In stock
Max:
Min: 1
Step: 1
B585-500 µg
500 µg
$429.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse B7-2 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:R380. The detection sensitivity of this indirect ELISA for Mouse B7-2 is approximately 1 ng/well.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing B7-2. A suggested method would be to stain cells expressing B7-2 with 0.25 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:R485.
Western Blotting: To detect Mouse B7-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for rmB7-2 is approximately 0.1 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse B7-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse B7-2 (at 10 ng/ml) is 2.5 - 10 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone GL-1 recognizes an epitope on mouse CD86.
Antigen Distribution
CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes.
Background
CD86 is an 80kD Ig superfamily member that is involved in immunoglobulin class-switching and activation of NK cell-mediated cytotoxicity. CD80 is closely related to, and works in tandem with CD86 to prime T- cells. CD86 is expressed earlier in the immune response than CD80. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD86 can also bind to CTLA-4 to deliver an inhibitory signal to T cells.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Indirect Elisa Protocol
Flow Cytometry
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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