Anti-Mouse CD86 – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C6158
Clone
GL1
Protein
B7-2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
B7-2, B70, Ly-58, CD-86
Isotype
Rat
IgG2a κ
Applications
B
,
ELISA
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C6158-1.0 mg
1.0 mg
$225.00
$175.50
In stock
Max:
Min: 1
Step: 1
C6158-5.0 mg
5.0 mg
$445.00
$347.10
In stock
Max:
Min: 1
Step: 1
C6158-25 mg
25 mg
$1,285.00
$1,002.30
In stock
Max:
Min: 1
Step: 1
C6158-50 mg
50 mg
$2,045.00
$1,595.10
In stock
Max:
Min: 1
Step: 1
C6158-100 mg
100 mg
$2,800.00
$2,184.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
LPS-activated CBA/Ca mouse splenic B cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISAThis antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
WB This antibody can be used to detect Human, Mouse and Rat TIM-1 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as PN:R951, under either reducing or non-reducing conditions. The positive control for Western blotting is PN:U124.
Other Applications Reported In Literature ?
B
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone GL-1 recognizes an epitope on mouse CD86.
Antigen Distribution
CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes.
Background
CD86 is an 80kD Ig superfamily member that is involved in immunoglobulin class-switching and activation of NK cell-mediated cytotoxicity. CD80 is closely related to, and works in tandem with CD86 to prime T- cells. CD86 is expressed earlier in the immune response than CD80. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD86 can also bind to CTLA-4 to deliver an inhibitory signal to T cells.

Antigen Details

Protein
Ligand/Receptor
CD28, CD152 (CTLA-4)
Function
T cell costimulation, Ig class-switching, NK cell cytotoxicity
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Costimulatory Molecules
.
Immunology
.
Neuroscience
.
Neuroscience Cell Markers

References & Citations

1. Hathcock, K.S. et al.. (1993) Science 262(5135:905-7
Elisa Sandwich Protocol
General Western Blot Protocol