Anti-Mouse CCL27 (Clone 68706)

Product No.: C1453


Clone 68706 Target CCL27 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names ALP, ILC, Eskine, CTACK, Chemokine (C-C Motif) Ligand 27 Isotype IgG2a Applications IHC FFPE , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen E. coli-Derived Recombinant Mouse CCL27 Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828516 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse CCL27 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for rmCCL27 is approximately 25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Immunohistochemistry: Suitable for use at concentration of 8-25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Rat Anti-Mouse CCL27 (Clone 68706) recognizes an epitope on Mouse CCL27. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues. PubMed CCL27 NCBI Gene Bank ID 10850 UniProt.org Information on Uniprot.org Research Area Chemokine References & Citations View product citations for antibody C1453 on CiteAb Technical Protocols IHC FFPE Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

E. coli-Derived Recombinant Mouse CCL27

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828516

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse CCL27 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for rmCCL27 is approximately 25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Additional Applications Reported In Literature ?

Immunohistochemistry: Suitable for use at concentration of 8-25 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse CCL27 (Clone 68706) recognizes an epitope on Mouse CCL27. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues.

PubMed

CCL27

NCBI Gene Bank ID

10850

UniProt.org

Information on Uniprot.org

Research Area

Chemokine

References & Citations

View product citations for antibody C1453 on CiteAb

IHC FFPE

Certificate of Analysis

Request COA

Prod No.	Description
C1409	Anti-Human CCL27 (Clone 124308)
C841	Recombinant Human CCL27
C1376	Anti-Human CCL27
C1410	Anti-Human CCL27 (Clone 124315)
C1453	Anti-Mouse CCL27 (Clone 68706)
C1452	Anti-Mouse CCL27 (Clone 68623)
C1475	Anti-Mouse CCL27/CTACK
C1605	Anti-Human CCL27 (Clone 124302)
C1494	Anti-Human CCL27-Biotin
C1520	Anti-Mouse CCL27 – Biotin

Formats Available

Prod No.	Description
C1453	Anti-Mouse CCL27 (Clone 68706)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Mouse CCL27 (Clone 68706)