Anti-Human CCL27 (Clone 124308)

Product No.: C1409


Clone 124308 Target CCL27 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Chemokine (C-C Motif) Ligand 27, CTACK, ALP, Skinkine, Eskine, ILC Isotype IgG1 Applications ELISA Indirect , IHC FFPE , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human CCL27 (Accession # NP_006655) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.8 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828467 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human CCL27 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL27 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C841. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human CCL27 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human CCL27 (at 1.0 ng/ml) is 1.5 - 4.5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human CCL27 recognizes an epitope on Human CCL27. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with the chemokines tested Background CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues. PubMed CCL27 Research Area Chemokine References & Citations View product citations for antibody C1409 on CiteAb Technical Protocols IHC FFPE N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human CCL27 (Accession # NP_006655)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.8 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828467

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human CCL27 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL27 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C841.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human CCL27 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human CCL27 (at 1.0 ng/ml) is 1.5 - 4.5 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human CCL27 recognizes an epitope on Human CCL27. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with the chemokines tested

Background

CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues.

PubMed

CCL27

Research Area

Chemokine

References & Citations

View product citations for antibody C1409 on CiteAb

IHC FFPE

Certificate of Analysis

Request COA

Prod No.	Description
C1409	Anti-Human CCL27 (Clone 124308)
C1376	Anti-Human CCL27
C1410	Anti-Human CCL27 (Clone 124315)
C1453	Anti-Mouse CCL27 (Clone 68706)
C1452	Anti-Mouse CCL27 (Clone 68623)
C1475	Anti-Mouse CCL27/CTACK
C1605	Anti-Human CCL27 (Clone 124302)
C841	Recombinant Human CCL27
C1520	Anti-Mouse CCL27 – Biotin
C1494	Anti-Human CCL27-Biotin

Formats Available

Prod No.	Description
C1409	Anti-Human CCL27 (Clone 124308)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human CCL27 (Clone 124308)