Anti-Human CCL27 (Clone 124308)

Pricing & Details

Product No.C1409
Clone
124308
Protein
CCL27
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Chemokine (C-C Motif) Ligand 27, CTACK, ALP, Skinkine, Eskine, ILC
Isotype
Mouse
IgG1
Applications
ELISA Indirect
,
IHC FFPE
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1409-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
C1409-100 µg
100 µg
$389.00
In stock
Max:
Min: 1
Step: 1
C1409-500 µg
500 µg
$849.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human CCL27 (Accession # NP_006655)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human CCL27 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL27 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C841.
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human CCL27 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CCL27 (at 1.0 ng/ml) is 1.5 - 4.5 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CCL27 recognizes an epitope on Human CCL27. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with the chemokines tested
Background
CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues.

Antigen Details

Protein
PubMed
Indirect Elisa Protocol
IHC
General Western Blot Protocol

Formats Available

Prod No.
Description
C1409
Products are for research use only. Not for use in diagnostic or therapeutic procedures.