Anti-Mouse CD11b – Purified (PhenoCyler-Fusion (CODEX)® Ready)

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Pricing & Details

Product No.C515
Clone
M1/70
Protein
CD11b
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Mac-1 αm chain
Isotype
Rat
IgG2b
Applications
CODEX®
,
IHC FF
Prod No.
Size
Price
Avail.
Qty
Add to cart
C515-50 µg
50 µg
$95.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

CODEX® Details

Validation Notes
Clone M1/70 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.
Tissue Screened
Spleen
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD11b (Clone M1/70) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2893572
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone M1/70 recognizes the α subunit (CD11b) of the mouse CD11b/CD18 complex.
Antigen Distribution
CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages.
Background
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.

Antigen Details

Protein
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
PubMed
NCBI Gene Bank ID

References & Citations

1. Kettenmann, H. et al. (2004) J Neurosci. 24(39):8500-9. PubMed
2. Jinquan, T. et al. (2006) J Immunol.177(6):3644-56. Article Link
3. Springer, T.A., et al. (1978) Eur. J. Immunol. 8:539
4. Springer, T.A., et al. (1982) Immunol. Rev. 68:171
5. Vignali, D. A. et al. (1990) J. Immunol. 144:4030
6. Kantor, A. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:3320
7. Jutila, M. A. et al. (1988) J. Immunol. 18:1819
IHC

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.