Anti-Mouse CD11b – Purified In vivo GOLD™ Functional Grade

Pricing & Details

  
Clone
M1/70
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Mac-1, Integrin αm chain, C3biR, CR3, Mo1, ITGAM
Isotype
Rat
IgG2b κ
Applications
CODEX®
,
CyTOF®
,
FA
,
FC
,
ICC
,
IHC FF
Prod No.
Size
Price
Avail.
Qty
Add to cart
C377-1.0 mg
1.0 mg
$95.00
In stock
C377-5.0 mg
5.0 mg
$298.00
In stock
C377-25 mg
25 mg
$775.00
In stock
C377-50 mg
50 mg
$1,275.00
In stock
C377-100 mg
100 mg
$1,998.00
In stock

Data

  

Antibody Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
B10 mouse spleen cells enriched for T cells
Product Concentration
≥ 2.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
FC The suggested concentration for this M1/70 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Functional Assay: Activation of mouse peritoneal macrophages3
Other Applications Reported In Literature ?
CyTOF®
CODEX®
ICC
IHC (Frozen)
Additional Reported Applications For Relevant Conjugates
B
Depletion
FA
FC
ICC
IHC (Frozen)
IHC (Paraffin)
IP
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications.

Description

Specificity
Clone M1/70 recognizes the α subunit (CD11b) of the mouse CD11b/CD18 complex.
Antigen Distribution
CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages.
Background
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.

Antigen Details

Protein
CD11b
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Function
Adhesion, chemotaxis
PubMed
cd11b m1/70
NCBI Gene Bank ID

References & Citations

1. Springer, T.A., et al. (1978) Eur. J. Immunol. 8:539 2. Springer, T.A., et al. (1982) Immunol. Rev. 68:171 3. Ding, A., et al. (1987) J Exp Med. 165:733 4. Vignali, D. A. et al. (1990) J. Immunol. 144:4030 5. Kantor, A. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:3320
Flow Cytometry
IHC
  

Formats Available

  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.