Anti-Mouse CD11b – FITC

Anti-Mouse CD11b – FITC

Product No.: C229

[product_table name="All Top" skus="C229"]

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Clone
M1/70
Target
CD11b
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Mac-1 αm chain
Isotype
IgG2b
Applications
FA
,
FC
,
ICC
,
IHC FF

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Select Product Size

Data

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Product Concentration
0.2 mg/ml
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Excitation Laser
Blue Laser (490 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this M1/70 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
ICC
IHC (Frozen)
Additional Reported Applications For Relevant Conjugates ?
Depletion
FA
IHC (Frozen)
IHC (Paraffin)
IP
IF Microscopy
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone M1/70 recognizes the α subunit (CD11b) of the mouse CD11b/CD18 complex.
Background
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
Antigen Distribution
CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages.
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
PubMed
NCBI Gene Bank ID
Research Area
Cell Adhesion
.
Cell Biology
.
Costimulatory Molecules
.
Immunology
.
Innate Immunity
.
Neuroscience
.
Neuroscience Cell Markers

References & Citations

1.) Springer, T.A., et al. (1978) Eur. J. Immunol. 8:539
2.) Springer, T.A., et al. (1982) Immunol. Rev. 68:171
3.) Ding, A., et al. (1987) J Exp Med. 165:733
4.) Vignali, D. A. et al. (1990) J. Immunol. 144:4030
5.) Kantor, A. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:3320
6.) Skyberg, J. A. et al. (2020) Infection and Immunity. 88: 5 Journal Link
FA
Flow Cytometry
ICC
IHC FF

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.