Anti-Mouse CD122 (IL-2Rβ) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD122 (IL-2Rβ) – Purified in vivo GOLD™ Functional Grade
Product No.: C2325
Clone TM-β1 Target CD122 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IL-2Rβ, Interleukin 2 receptor β chain, IL-2/15Rb Isotype Rat IgG2b κ Applications B , Depletion , FC , in vivo , IP , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Rat T-cell line expressing Mouse IL-2Rβ Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737464 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this TM-β1 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this TM-β1 antibody for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ? B IP Depletion Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone TM-β1 recognizes an epitope on mouse CD122. Background CD122 is a 70-75 kD IL-2 receptor β chain that is a type I membrane protein. CD122 is involved in T cell-mediated immune responses and its activation increases proliferation of CD8+ effector T cells. It exists in three forms with varying degrees of binding affinity with IL-2. The low affinity form is a monomer of the α subunit and has no involvement in signal transduction. The intermediate affinity form is a γ/β heterodimer and the high affinity form is an α/β/γ heterotrimer. The intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. This protein also interacts with the IL-15 receptor. Antigen Distribution CD122 is expressed on NK cells and at lower levels by T lymphocytes, B lymphocytes, monocytes, and macrophages. Ligand/Receptor IL-2, IL-15 Function Critical component of IL-2 and IL-15 signaling PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone TM-β1 (TM-?1) is a monoclonal antibody targeting mouse CD122 (IL-2/IL-15 receptor β chain) and is extensively used in in vivo mouse studies to block signaling through the IL-2 and IL-15 pathways. This blockade is strategically employed to:
Key details:
In summary, clone TM-β1 is a functional tool for in vivo immunological studies, enabling precise blocking of IL-2/IL-15 signaling and depletion of target immune cells, with widespread utility in mouse models of immunology and therapy research. Commonly used antibodies or proteins that are co-used with TM-?1 (assuming you refer to a transmembrane protein such as TMEM family proteins, or a generic "TM-1" antibody) in the literature typically depend on the application and biological context, but several categories are prevalent:
Example combinations:
If you have a specific TM-?1 protein or context in mind, please clarify, as the most appropriate co-validated antibodies can be highly context-dependent. The examples above reflect the general literature trends for transmembrane and antibody studies. There seems to be a lack of clear information about "clone TM-?1" in the scientific literature provided. However, I can clarify findings related to Tm-1 and a monoclonal antibody called Clone TMβ1. Tm-1 Findings:
Clone TMβ1 (Anti-Mouse CD122):
There is no specific detailed citation analysis or set of findings directly associated with "clone TM-?1" in the provided search results. If you are looking for specific aspects of these clones, further clarification might help in providing a more precise answer. Dosing Regimens of Clone TM-β1 (Anti-CD122, IL-2Rβ) in Mouse ModelsClone TM-β1 (often referred to as "TM-β1," but sometimes written as "TM-?1" due to typographical ambiguity) is a monoclonal antibody targeting the CD122 chain of the IL-2 receptor (IL-2Rβ) and is used in immunology research to deplete or block specific immune cell populations, such as regulatory T cells (Tregs) or natural killer (NK) cells, in mouse models. Variation Across Mouse Models
Summary Table
Key Points
In summary, while TM-β1 is a valuable tool for immune cell depletion in mice, its dosing regimen is highly dependent on the specific model and experimental goals. Researchers should carefully optimize and validate their protocol based on the available literature and preliminary data for their particular application. References & Citations1. Burchill, MA. et al. (2007) J. Immunol. 178:280 2. Friedmann, MC. et al. (1996) Proc. Natl. Acad. Sci. (USA) 93:2077 3. Leonard, WJ. et al. (1987) Science 238:75 Technical ProtocolsCertificate of Analysis |
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Formats Available
