Anti-Mouse CD122 (IL-2Rβ) – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2325
Clone
TM-β1
Protein
CD122
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL-2Rβ, Interleukin 2 receptor β chain, IL-2/15Rb
Isotype
Rat
IgG2b κ
Applications
B
,
Depletion
,
FC
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2325-0.5 mg
0.5 mg
$175.00
In stock
Max:
Min: 1
Step: 1
C2325-1.0 mg
1 mg
$355.00
In stock
Max:
Min: 1
Step: 1
C2325-5.0 mg
5 mg
$998.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Rat T-cell line expressing Mouse IL-2Rβ
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this TM-β1 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this TM-β1 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
B
IP
Depletion
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone TM-β1 recognizes an epitope on mouse CD122.
Antigen Distribution
CD122 is expressed on NK cells and at lower levels by T lymphocytes, B lymphocytes, monocytes, and macrophages.
Background
CD122 is a 70-75 kD IL-2 receptor β chain that is a type I membrane protein. CD122 is involved in T cell-mediated immune responses and its activation increases proliferation of CD8+ effector T cells. It exists in three forms with varying degrees of binding affinity with IL-2. The low affinity form is a monomer of the α subunit and has no involvement in signal transduction. The intermediate affinity form is a γ/β heterodimer and the high affinity form is an α/β/γ heterotrimer. The intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. This protein also interacts with the IL-15 receptor.

Antigen Details

Protein
Ligand/Receptor
IL-2, IL-15
Function
Critical component of IL-2 and IL-15 signaling
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Burchill, MA. et al. (2007) J. Immunol. 178:280
2. Friedmann, MC. et al. (1996) Proc. Natl. Acad. Sci. (USA) 93:2077
3. Leonard, WJ. et al. (1987) Science 238:75
Flow Cytometry
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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