Anti-Mouse CD127 (IL-7Rα) – DyLight® 488

Anti-Mouse CD127 (IL-7Rα) – DyLight® 488

Product No.: C2229

[product_table name="All Top" skus="C2229"]

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Clone
A7R34
Target
CD127
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL-7Rα, Interleukin-7 receptor alpha, IL-7Rα chain
Isotype
IgG2a κ
Applications
FC

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse CD127 (>98%)
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (493 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this A7R34 antibody for staining cells in flow cytometry is ≤ 2.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone A7R34 recognizes an epitope on mouse CD127.
Antigen Distribution
IL-7Rα is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, NK-cells, naïve T cells, memory CD4+ and CD8+ T cells, intestinal colorectal and renal carcinoma cell lines, and keratinocytes.
Background
CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/γ chain heterodimer. The common γ chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells.

Antigen Details

Ligand/Receptor
IL-7
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Fry TJ et al. (2005) J Immunol. 174: 6571 2. Murre C et al. (2002) Embo J. 21: 103
Flow Cytometry

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.