Anti-Mouse CD127 (IL-7Rα) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD127 (IL-7Rα) – Purified in vivo GOLD™ Functional Grade
Product No.: C2223
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Clone A7R34 Target CD127 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IL-7Rα, Interleukin-7 receptor alpha, IL-7Rα chain Isotype Rat IgG2a κ Applications B , CyTOF® , FA , FC , IF Staining , IHC FF , in vivo , IP , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen IL-7Ra-IgG1 fusion protein Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737461 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this A7R34 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? B CyTOF® FA IF Staining IHC (Frozen) IP WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone A7R34 recognizes an epitope on mouse CD127. Background CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/γ chain heterodimer. The common γ chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells. Antigen Distribution IL-7Rα is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, NK-cells, naïve T cells, memory CD4+ and CD8+ T cells, intestinal colorectal and renal carcinoma cell lines, and keratinocytes. Ligand/Receptor IL-7 Function T cell and immature B cell proliferation and development PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Fry TJ et al. (2005) J Immunol. 174: 6571
2. Murre C et al. (2002) Embo J. 21: 103 Technical ProtocolsB CyTOF® FA  IF Staining IHC FF    Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
