Anti-Mouse CD127 (IL-7Rα) – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2223
Clone
A7R34
Protein
CD127
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL-7Rα, Interleukin-7 receptor alpha, IL-7Rα chain
Isotype
Rat
IgG2a κ
Applications
B
,
CyTOF®
,
FA
,
FC
,
IF Staining
,
IHC FF
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2223-0.5 mg
0.5 mg
$170.00
In stock
Max:
Min: 1
Step: 1
C2223-1.0 mg
1.0 mg
$245.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
IL-7Ra-IgG1 fusion protein
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this A7R34 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
B
CyTOF®
FA
IF Staining
IHC (Frozen)
IP
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone A7R34 recognizes an epitope on mouse CD127.
Antigen Distribution
IL-7Rα is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, NK-cells, naïve T cells, memory CD4+ and CD8+ T cells, intestinal colorectal and renal carcinoma cell lines, and keratinocytes.
Background
CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/γ chain heterodimer. The common γ chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells.

Antigen Details

Protein
Ligand/Receptor
IL-7
Function
T cell and immature B cell proliferation and development
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Fry TJ et al. (2005) J Immunol. 174: 6571 2. Murre C et al. (2002) Embo J. 21: 103
Flow Cytometry
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available