Anti-Mouse CD18 (Clone C71/16) – Purified in vivo PLATINUM™ Functional Grade

Clone C71/16 is a rat monoclonal antibody specific for mouse CD18 (the ?2 integrin); in in vivo mouse studies, it is primarily used to functionally block or detect CD18 on leukocytes, thereby allowing researchers to study the role of CD18 in immune cell adhesion, migration, and signaling.

Key uses in vivo include:

• Functional Blocking: Clone C71/16 is deployed to inhibit ?2 integrin function (CD18), disrupting leukocyte adhesion and migration, as part of experiments analyzing the contribution of CD18 to various immune responses or disease models.
• Cell Signaling and Manipulation: In vivo grade C71/16 antibodies are used to manipulate biological systems—for example, by blocking immune cell trafficking, which is vital for studying inflammation, autoimmunity, or pathogen responses in mouse models.
• Preclinical Validation: The antibody is validated for functional assays in mice and is produced under conditions minimizing endotoxin and pathogen contamination, ensuring safety and reliability for in vivo administration.
• Staining and Detection: While C71/16 is also used for flow cytometric analysis (staining mouse leukocytes ex vivo), its in vivo use focuses on direct modulation of CD18 signaling.

Additional context:

• The antibody is often administered via intraperitoneal or intravenous injection, depending on experimental protocol, and dosed based on the effect required—either blocking or partial inhibition of CD18.
• Functional grade preparations, such as Leinco’s PLATINUM™, are specifically tested for low endotoxin and absence of mouse pathogens, as contamination could confound immune studies in live mice.
• Example applications in published studies include blocking CD18 to assess its role in leukocyte migration in inflammation, infection, tumor immunity, and other pathophysiological processes.

In summary, clone C71/16 is a critical tool in mouse research for in vivo functional modulation of CD18, primarily to investigate immune cell behaviors and integrin-mediated processes.

The correct storage temperature for sterile packaged clone C71/16 depends on its biological nature (e.g., live cells, bacterial cultures, DNA, or a medical device). If you are referring to a biological clone or cell line, such materials are typically stored as follows:

• For long-term storage: Ultra-low freezer at -80°C is standard for most biological clones, especially if they are living cells, as this preserves viability and genetic stability.
• If cryopreserved (in liquid nitrogen): Storage at -150°C to -190°C is used for the most sensitive or high-value samples, such as stem cells, hybridomas, or certain primary cell lines.

If C71/16 is a sterile packaged medical device (not a live biological material), best practice is:

• Store at a maximum room temperature of 25°C,
• In a dry, clean, protected from light and dust environment,
• As specified by the packaging’s use-by date and hygiene protocols.

Essential Details:

• Cells/Clones:
  • Short-term: may be kept at 2–8°C for days if recently thawed, but -80°C or lower is required for long-term integrity.
  • Never store live cells at room temperature for long-term or at -20°C, as viability will be lost.
• Sterile Medical Devices:
  • Max storage temperature: 25°C, in a clean, monitored storage area, to maintain sterility.
  • Use-by dates are only valid if these storage conditions are continuously maintained.

If the identity or nature of 'clone C71/16' is not confirmed in your materials, consult the supplier’s datasheet for precise storage instructions. Most molecular biology and cell culture clones are stored at -80°C or below, except if in DNA form (plasmids, etc.), which may sometimes be stable at -20°C or 2–8°C for short periods.

If you provide the exact product type or supplier, a more precise temperature recommendation can be given.

Antibodies commonly used with C71/16 (anti-mouse CD18) in the literature are those targeting the individual ? chains that pair with CD18, notably antibodies to CD11a, CD11b, and CD11c, as well as other immune cell markers such as CD3, CD4, and CD8.

CD18 is the ?2 integrin subunit that forms heterodimers with various ? chains (CD11a, CD11b, CD11c), creating LFA-1 (CD11a/CD18), Mac-1 (CD11b/CD18), and p150,95 (CD11c/CD18). Antibodies against these ? chains (CD11a, CD11b, CD11c) are frequently used alongside C71/16 to differentiate between integrin complexes and to study their roles in immune cell adhesion and migration. These combinations are standard in immunophenotyping panels to map leukocyte populations or investigate cell adhesion mechanisms.

Other antibodies that are commonly paired with C71/16 in multi-color flow cytometry or immunohistochemistry panels include:

• CD3, CD4, CD8: To identify T cell subsets.
• CD45 and CD14: For broader leukocyte profiling and monocyte identification.
• Gr-1 (Ly6G/Ly6C): For granulocyte detection.

These markers allow researchers to analyze the expression of CD18 on specific immune cell types and study its functional role in conjunction with other integrins and adhesion molecules.

Experiments using C71/16 often employ:

• Isotype controls (Rat IgG2a)
• Various conjugated forms (e.g., FITC, biotin) for compatibility in multiplex assays

Finally, in studies focusing on integrin-mediated adhesion, researchers may use antibodies against ICAM-1 and VCAM-1 to assess interactions with CD18-containing integrins.

In summary, anti-CD11a, CD11b, CD11c antibodies are the most commonly co-used with C71/16, followed by markers for immune cell subset identification and isotype controls for assay calibration.

The key findings from scientific literature citing clone C71/16 center on its use as a monoclonal antibody for detecting the murine integrin CD11b (also known as Mac-1, part of the CD11b/CD18 complex) in immunological research. This clone has played a crucial role in elucidating several mechanisms related to immune cell behavior, especially in the context of inflammation, leukocyte trafficking, and resolution of immune responses.

Highlights from C71/16 citations:

• Identification and Quantification of CD11b: C71/16 has been instrumental in identifying and quantifying CD11b expression on various leukocyte populations, allowing researchers to study its dynamic regulation during inflammation and immune responses.

• Integrin Shedding and Immune Regulation: Studies using C71/16 contributed to characterizing the metalloproteinase-mediated shedding of integrin ?2 (CD18) and how it modulates macrophage efflux from inflammatory sites, thereby influencing resolution of inflammation.

• Functional Differentiation During Arthritis: C71/16 has enabled the detection of shed CD11b/CD18 complexes in synovial fluid, which distinguished forms of arthritis (e.g., rheumatoid arthritis vs. other types) via unique epitope presentation, reflecting different inflammatory pathways.

• Role in Macrophage and Neutrophil Studies: The antibody has been used to monitor the presence and function of macrophages and neutrophils, especially the efflux of macrophages to lymphatics and changes during efferocytosis (clearance of apoptotic cells), linking surface marker changes to functional states.

• Modeling Defective Immune Responses: Research employing C71/16 has included studies of CD18 null mice, showing how loss of Mac-1 leads to spontaneous skin ulceration and defective T cell function—highlighting the importance of the integrin for immune interactions.

Keywords associated with clone C71/16 in literature:

• CD11b detection
• Mac-1 integrin analysis
• Leukocyte migration
• Inflammation resolution
• Arthritis differentiation
• Macrophage efflux

C71/16 is recognized as a vital experimental tool for immunophenotyping and dissecting the molecular mechanisms underlying immune cell adhesion, migration, and inflammation in murine models. No conflicting results about its specificity or utility were found among the cited scientific sources.