Anti-Mouse CD18 (Clone C71/16) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse CD18 (Clone C71/16) – Purified in vivo PLATINUM™ Functional Grade

Product No.: C678

[product_table name="All Top" skus="C378"]

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Clone
C71/16
Target
CD18
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Integrin beta-2, Mac-1 beta, macrophage antigen-1 beta, complement receptor C3 subunit beta, lymphocyte function associated antigen 1, cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, 2E6, LAD, Cd18, Lfa1, MF17, LCAMB, AI528527
Isotype
Rat IgG2a
Applications
FA
,
in vivo
,
IP
,
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Recommended Isotype Controls
Recommended Dilution Buffer
Immunogen
Cell membrane glycoproteins from mouse T-cell lymphoma BW5147
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this C71/16 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this C71/16 antibody for use in western blotting is 1-10 μg/ml.
Additional Applications Reported In Literature ?
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone C71/16 recognizes an epitope on mouse CD18.
Background
CD18 is a 95kD type I transmembrane glycoprotein. CD18 is the Integrin β2 chain and forms multiple heterodimers with the three integrin α chains, CD11a, CD11b, and CD11c. CD18 binds with CD11a to form LFA-1. LFA-1 is involved in adhesion and binding to antigen presenting cells via interactions with ICAM-1. CD18 binds with CD11b and CD11c forming complement receptors , such as MAC-1, that participate in the innate immune response by recognizing foreign antigen peptides and destroying them via phagocytosis. In humans, lack of functional CD18 causes Leukocyte Adhesion Deficiency (LAD) which is characterized by the inability of circulating leukocytes to respond to foreign bodies, reducing the ability of the immune system to fight off infection.
Antigen Distribution
CD18 is present on mouse T-cells, B-cells, NK cells, monocytes, macrophages and granulocytes.
PubMed
NCBI Gene Bank ID
Research Area
Cell Adhesion
.
Cell Biology
.
Immunology
.
Innate Immunity

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Commonly used antibodies or proteins studied alongside C71/16 (anti-CD18, integrin β2) in the literature typically include those that recognize the α-chains (CD11a, CD11b, CD11c), as well as markers for immune cell identification and functional assays. This is because CD18 is the shared β-subunit that pairs with various α-integrins to form important leukocyte adhesion molecules.

Key antibodies or proteins commonly used with C71/16 include:

  • Anti-CD11a (LFA-1, αL integrin): Forms the LFA-1 complex with CD18. Frequently used to assess the αLβ2 integrin function in leukocyte adhesion and migration.
  • Anti-CD11b (Mac-1, αM integrin): Forms Mac-1 (αMβ2) with CD18, playing a role in phagocytosis, cell migration, and inflammation.
  • Anti-CD11c (αX integrin): Forms p150,95 (αXβ2), particularly important in dendritic cell studies.
  • Immune cell subset markers: Antibodies against CD3 (T cells), CD19 (B cells), CD45 (pan-leukocyte), and other lineage-specific markers are typically included in multicolor flow cytometry panels to define which cells express integrins.
  • Fc block reagents: Such as anti-CD16/CD32 (FcγRII/III), are commonly used in protocols to reduce non-specific binding before staining for CD18 and related markers.

Relevant published panels often use:

  • Fluorescently conjugated anti-CD18 (such as C71/16-FITC), anti-CD11a-PE, anti-CD11b-APC, and markers for T cells or myeloid cells for detailed leukocyte profiling by flow cytometry.

Summary table:

TargetPurpose/ContextExample Clone or Info
CD11a (αL integrin)LFA-1 complex, T cell adhesion studiesOften clone M17/4
CD11b (αM integrin)Mac-1 complex, myeloid cell identificationCommon clone M1/70
CD11c (αX integrin)Dendritic cell and myeloid studiesVarious clones (e.g., N418)
CD3, CD19, CD45, etc.Lineage and immune subset discriminationMany standard clones
Fc block (CD16/32)Prevent non-specific bindingClone 2.4G2
Viability dyesExclude dead cells in FACS-

The combination of C71/16 with CD11a, CD11b, CD11c, and cell lineage markers is extensively reported in studies examining immune integrin biology, cell adhesion, and leukocyte function.

If a more targeted list of published panels or specific combinations is required, referencing literature in immunology, particularly in flow cytometry or functional adhesion assays, will yield representative examples where these antibodies are used together.

The clone C71/16 is referenced in relation to Rat anti-CD18 PE antibodies, which are typically used in research involving the study of integrins and their role in cell adhesion and immune responses. The main scientific literature mentioning this clone would likely focus on its application in experiments related to CD18, an important component of the integrin family, particularly in the context of immune cell interactions and inflammation.

While specific key findings directly from clone C71/16 citations are not detailed in the provided search results, the study of CD18 and its related antibodies often pertains to understanding its role in:

  • Immune Cell Function: CD18 is crucial for the functioning of various immune cells, including neutrophils and macrophages, impacting their adhesion and migration.
  • Inflammation: Alterations in CD18 expression or function can affect inflammatory responses, influencing conditions like autoimmunity and inflammation-related diseases.
  • Experimental Models: The use of antibodies like Rat anti-CD18 PE (clone C71/16) helps in characterizing and analyzing immune cell behaviors in experimental setups.

For detailed insights into specific findings related to clone C71/16, reviewing articles that utilize this antibody for research purposes would be necessary.

Dosing regimens for clone C71/16 can vary considerably depending on the specific mouse model and experimental objectives, but there is currently no publicly available data in the provided search results that indicates preferred or standard regimens for this particular antibody clone in mice.

  • Most search results, including comprehensive dosing guides for common antibody clones, do not mention C71/16 or its recommended doses across mouse models.
  • Other referenced sources relate broadly to dosing of small molecules or other biological agents in mice, but none cite C71/16 by name.

For other clones, typical regimens in mice (for immunotherapy or depletion studies) tend to fall in the range of 100–250 μg per mouse per dose, administered intraperitoneally every 2–3 days or 2–3 times per week. These schedules are optimized according to antibody target, mouse strain, disease model, and experimental endpoints.

If C71/16 is a novel or less commonly used clone, researchers usually:

  • Refer to manufacturer or literature-based pilot doses.
  • Adjust regimens based on pharmacodynamics (e.g., biomarker response) or toxicity.

Key factors affecting dosing in different mouse models:

  • Strain differences (e.g., immunodeficient vs. immunocompetent)
  • Disease model (tumor, autoimmune, infection, depletion studies)
  • Age and weight of mice
  • Route of administration (typically intraperitoneal for antibodies)

Summary: The optimal dosing regimen for clone C71/16 across mouse models is not documented in the provided search results. In the absence of direct literature, researchers should use dosing analogies from similar antibody studies (100–250 μg per mouse, IP, 2–3x/week), and titrate according to specific experimental requirements, referencing primary data where available.

If you need specifics for C71/16, I recommend consulting proprietary datasheets, contacting the manufacturer, or searching primary research publications for direct regimens.

References & Citations

Springer, T. et al. (1978) Eur. J. Immunol. 8:539
FA
in vivo Protocol
Immunoprecipitation Protocol
General Western Blot Protocol

Certificate of Analysis

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.