Anti-Mouse CD18 (Clone C71/16) – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD18 (Clone C71/16) – Purified in vivo PLATINUM™ Functional Grade
Product No.: C678
Clone C71/16 Target CD18 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Integrin beta-2, Mac-1 beta, macrophage antigen-1 beta, complement receptor C3 subunit beta, lymphocyte function associated antigen 1, cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, 2E6, LAD, Cd18, Lfa1, MF17, LCAMB, AI528527 Isotype Rat IgG2a Applications FA , in vivo , IP , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Cell membrane glycoproteins from mouse T-cell lymphoma BW5147 Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2893577 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this C71/16 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this C71/16 antibody for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ? IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone C71/16 recognizes an epitope on mouse CD18. Background CD18 is a 95kD type I transmembrane glycoprotein. CD18 is the Integrin β2 chain and forms multiple heterodimers with the three integrin α chains, CD11a, CD11b, and CD11c. CD18 binds with CD11a to form LFA-1. LFA-1 is involved in adhesion and binding to antigen presenting cells via interactions with ICAM-1. CD18 binds with CD11b and CD11c forming complement receptors , such as MAC-1, that participate in the innate immune response by recognizing foreign antigen peptides and destroying them via phagocytosis. In humans, lack of functional CD18 causes Leukocyte Adhesion Deficiency (LAD) which is characterized by the inability of circulating leukocytes to respond to foreign bodies, reducing the ability of the immune system to fight off infection. Antigen Distribution CD18 is present on mouse T-cells, B-cells, NK cells, monocytes, macrophages and granulocytes. PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Commonly used antibodies or proteins studied alongside C71/16 (anti-CD18, integrin β2) in the literature typically include those that recognize the α-chains (CD11a, CD11b, CD11c), as well as markers for immune cell identification and functional assays. This is because CD18 is the shared β-subunit that pairs with various α-integrins to form important leukocyte adhesion molecules. Key antibodies or proteins commonly used with C71/16 include:
Relevant published panels often use:
Summary table:
The combination of C71/16 with CD11a, CD11b, CD11c, and cell lineage markers is extensively reported in studies examining immune integrin biology, cell adhesion, and leukocyte function. If a more targeted list of published panels or specific combinations is required, referencing literature in immunology, particularly in flow cytometry or functional adhesion assays, will yield representative examples where these antibodies are used together. The clone C71/16 is referenced in relation to Rat anti-CD18 PE antibodies, which are typically used in research involving the study of integrins and their role in cell adhesion and immune responses. The main scientific literature mentioning this clone would likely focus on its application in experiments related to CD18, an important component of the integrin family, particularly in the context of immune cell interactions and inflammation. While specific key findings directly from clone C71/16 citations are not detailed in the provided search results, the study of CD18 and its related antibodies often pertains to understanding its role in:
For detailed insights into specific findings related to clone C71/16, reviewing articles that utilize this antibody for research purposes would be necessary. Dosing regimens for clone C71/16 can vary considerably depending on the specific mouse model and experimental objectives, but there is currently no publicly available data in the provided search results that indicates preferred or standard regimens for this particular antibody clone in mice.
For other clones, typical regimens in mice (for immunotherapy or depletion studies) tend to fall in the range of 100–250 μg per mouse per dose, administered intraperitoneally every 2–3 days or 2–3 times per week. These schedules are optimized according to antibody target, mouse strain, disease model, and experimental endpoints. If C71/16 is a novel or less commonly used clone, researchers usually:
Key factors affecting dosing in different mouse models:
Summary: The optimal dosing regimen for clone C71/16 across mouse models is not documented in the provided search results. In the absence of direct literature, researchers should use dosing analogies from similar antibody studies (100–250 μg per mouse, IP, 2–3x/week), and titrate according to specific experimental requirements, referencing primary data where available. If you need specifics for C71/16, I recommend consulting proprietary datasheets, contacting the manufacturer, or searching primary research publications for direct regimens. References & CitationsSpringer, T. et al. (1978) Eur. J. Immunol. 8:539 Technical ProtocolsCertificate of Analysis |
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