Anti-Mouse CD28 [Clone 37.51] — Purified in vivo PLATINUM™ Functional Grade

C57BL/6 mouse T-cell lymphoma EL-4

This monoclonal antibody is aseptically packaged and formulated in 20 mM L-histidine and 150 mM NaCl buffer, pH 5.9-6.1 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

Clone 37.51 recognizes an epitope on mouse CD28.

CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.

CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.

CD80 (B7-1), CD86 (B7-2)

1. Gallimore, A. et al. (2005) J Immunol. 175(11):7098-102. PubMed
2. Gross, J. A. et al. (1992) J. Immunol. 149:380