Anti-Mouse CD28 – Biotin

Pricing & Details

  
Clone
37.51
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
MGC138290, Tp44
Isotype
Syrian Hamster
IgG
Applications
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
C244-100 µg
100 µg
$70.00
In stock
C244-500 µg
500 µg
$220.00
In stock
  

Antibody Details

Reactivity Species
Mouse
Host Species
Syrian Hamster
Product Concentration
0.5 mg/ml
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
FC The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD28. A suggested method would be to stain cells expressing CD28 with ≤0.5 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:A104.
Additional Reported Applications For Relevant Conjugates
B
Cosim
IP
IHC (Frozen)
For specific conjugates of this clone, review literature for suggested application details.

Description

Specificity
Clone 37.51 recognizes an epitope on mouse CD28.
Antigen Distribution
CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.
Background
CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.

Antigen Details

Protein
CD28
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
PubMed
anti-mouse cd28
NCBI Gene Bank ID

References & Citations

Gross, J. A. et al. (1992) J. Immunol. 149:380
Flow Cytometry
  

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.