Anti-Mouse CD28 – Purified

Pricing & Details

  
Clone
37.51
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
MGC138290, Tp44
Isotype
Syrian Hamster
IgG
Applications
B
,
Costim
,
FC
,
IHC FF
,
IP
Prod No.
Size
Price
Avail.
Qty
Add to cart
C243-50 µg
50 µg
$45.00
In stock
C243-500 µg
500 µg
$185.00
In stock

Data

  

Antibody Details

Reactivity Species
Mouse
Host Species
Syrian Hamster
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
FC The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Applications and Recommended Usage ?
(Quality Tested by Leinco)

Other Applications Reported In Literature ?
IHC (Frozen)
B
Cosim
IP
Each investigator should determine their own optimal working dilution for specific applications.

Description

Specificity
Clone 37.51 recognizes an epitope on mouse CD28.
Antigen Distribution
CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.
Background
CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.

Antigen Details

Protein
CD28
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
PubMed
anti-mouse cd28
NCBI Gene Bank ID

References & Citations

Gross, J. A. et al. (1992) J. Immunol. 149:380
Flow Cytometry
IHC
Immunoprecipitation Protocol
  

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.