Anti-Mouse CD28 – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C379
Clone
37.51
Protein
CD28
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
MGC138290, Tp44, T44
Isotype
Syrian Hamster
IgG
Applications
B
,
Costim
,
CyTOF®
,
FC
,
IHC FF
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C379-1.0 mg
1.0 mg
$105.00
$81.90
In stock
Max:
Min: 1
Step: 1
C379-5.0 mg
5.0 mg
$355.00
$276.90
In stock
Max:
Min: 1
Step: 1
C379-25 mg
25 mg
$965.00
$752.70
In stock
Max:
Min: 1
Step: 1
C379-50 mg
50 mg
$1,595.00
$1,244.10
In stock
Max:
Min: 1
Step: 1
C379-100 mg
100 mg
$2,300.00
$1,794.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Syrian Hamster
Immunogen
C57BL/6 mouse T-cell lymphoma EL-4
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this 37.51 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
CyTOF®
IHC (Frozen)
B
Cosim
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 37.51 recognizes an epitope on mouse CD28.
Antigen Distribution
CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.
Background
CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.

Antigen Details

Protein
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Function
Costimulates T and NK cells
PubMed
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
.
Immunology

References & Citations

1. Gallimore, A. et al. (2005) J Immunol. 175(11):7098-102. PubMed
2. Gross, J. A. et al. (1992) J. Immunol. 149:380
Flow Cytometry
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available