Anti-Mouse CD3ε – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Mouse CD3ε – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C523

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Clone
145-2C11
Target
CD3ε
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD3, T3
Isotype
IgG
Applications
IHC FF
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone 145-2C11 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.
Tissue Screened
Spleen
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Concentration
0.5 mg/ml
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD3ε (Clone 145-2C11) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2892846
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 145-2C11 recognizes an epitope on mouse CD3ε.
Antigen Distribution
CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein
Ligand/Receptor
Peptide antigen/MHC-complex
PubMed
NCBI Gene Bank ID
IHC
PhenoCycler®
Products are for research use only. Not for use in diagnostic or therapeutic procedures.