Anti-Mouse CD90 (Thy-1) (Clone HK2.1) – Purified in vivo PLATINUM™ Functional Grade

The clone HK2.1 is a monoclonal antibody used in in vivo mouse studies to target and recognize CD90 (Thy-1) alloantigens, specifically both Thy-1.1 and Thy-1.2, which are present on most mouse strains.

Key uses and features in in vivo mouse studies:

• Functional antibody for in vivo use: HK2.1 is supplied as a functional-grade antibody, suitable for direct use in live animal experiments, not just for in vitro assays.
• Cell targeting and depletion: Because CD90 is broadly expressed on hematopoietic stem cells, thymocytes, peripheral T cells, some B cells, neurons, endothelial and other cell types, clone HK2.1 is often used for:
  • Identifying, labeling, or depleting specific cell populations bearing CD90 in mice during immune, stem cell, or neurobiology studies.
  • Studying T cell biology, as CD90 is involved in T cell activation, proliferation, and adhesion to thymic stroma.
• Isotype and purification: HK2.1 is a rat IgG2c antibody and is purified with process steps to ensure low endotoxin, making it especially suitable for experiments involving live animals (to minimize off-target inflammation or reactions).
• Alloantigen recognition: HK2.1 binds a non-polymorphic epitope present on both the Thy1.1 and Thy1.2 variants, making it universally applicable across commonly used mouse strains for tracking CD90+ cells.

Modes of application:

• In vivo antibody treatment: Direct administration to mice—for example, for functional blocking, immune cell depletion, or tracking of CD90+ populations. The exact dosing and protocol details are normally found on the product datasheet or scientific literature corresponding to specific studies.
• Flow cytometry and functional assays: While not exclusive to in vivo, the same clone may be used for ex vivo analysis by flow cytometry (FACS) of cells harvested from treated animals.

Summary Table: Clone HK2.1 in In Vivo Mouse Studies

No direct information was found in the results about specific disease models or exact dosing protocols; these are usually defined by the experimental design and available from manufacturers or in primary literature for each indicated use. The antibody’s broad specificity and low endotoxin content make it suitable for a variety of applications in mouse immunology and stem cell research.

For sterile packaged clone HK2.1 (a cell line—likely derived from the proximal tubule HK2—common in cell biology research), the correct storage temperature is below -130°C, preferably in the vapor phase of liquid nitrogen, until ready for use. This is the standard for long-term cryopreservation of most cultured mammalian cells and is recommended to maintain maximum cell viability.

Key points:

• Immediate transfer upon receipt: Move the frozen vial from dry ice directly to a storage environment below -130°C, ideally the vapor phase of liquid nitrogen, to ensure temperature stability.
• Short-term storage: If liquid nitrogen is not available, storage at -80°C is possible for up to two weeks, but this is less ideal as viability may decline unpredictably with time.
• Upon thawing: Always quickly thaw cells at 37°C using a water bath, and handle under sterile conditions.

Do not store cells at temperatures above -80°C for extended periods, as this will significantly reduce viability and may result in loss of the clone. For immediate use, follow the established thawing and culturing protocols.

Summary Table

Conclusion:
For sterile packaged clone HK2.1, the correct storage temperature is below -130°C in liquid nitrogen vapor phase until ready for use. Short-term storage at -80°C is acceptable if absolutely necessary, but viability will decline over time.

Commonly used antibodies or proteins with HK2.1 in the literature include a variety of metabolic enzymes, stem cell markers, and housekeeping proteins for controls. The following are among the most frequently co-used antibodies and proteins in studies with HK2:

• Lin28 (stem cell marker)
• Nanog (stem cell marker)
• PDK1 (pyruvate dehydrogenase kinase 1)
• PGK1 (phosphoglycerate kinase 1)
• PFKP (platelet-type phosphofructokinase)
• COX IV (cytochrome c oxidase subunit IV)
• PKM2 (pyruvate kinase M2)
• Oct4 (stem cell marker)
• HA, GST, Flag (tag antibodies for labeled proteins)
• Ubiquitin (protein degradation pathway)
• CD133 (cancer stem cell surface marker)
• GLUT1 (glucose transporter 1)
• LDHA (lactate dehydrogenase A)
• USP11 (ubiquitin-specific peptidase 11)
• Tubulin, GAPDH (loading controls for western blot/immunofluorescence)

These proteins are chosen because they represent controls, markers of cell state (such as stemness or metabolic activity), or are mechanistically linked to HK2’s role in cellular metabolism and cancer biology. Researchers commonly use these antibodies in western blot and immunofluorescence to study HK2 interactions, localization, and expression profile.

Additionally, HK1 is often studied alongside HK2 to distinguish isoform-specific effects in metabolic studies or genetic models. When tags or genetic manipulations are employed, HA, Flag, or GST tag antibodies are used for detection of exogenously expressed or purified proteins.

Experimental controls in these setups regularly include antibodies against tubulin and GAPDH to verify equal protein loading on gels or in immunostaining experiments.

This reflects a broad approach in literature, where the study of HK2.1 typically intersects with cell metabolism, stemness, and cellular stress proteins.

The key findings from citations mentioning clone HK2.1 in scientific literature are not directly available in the provided search results. There are no references to a "clone HK2.1" as a scientific reagent, cell line, or antibody in the cited articles. Instead, the results address topics related to the enzyme hexokinase II (HK2) and the protein human Kallikrein 2 (hK2), but without explicit mention of a "clone HK2.1."

Essential context:

• HK2 (hexokinase II) is a metabolic enzyme frequently studied in cancer research. One study performed a pan-cancer analysis of HK2 and reported its varied expression and genetic alterations across different tumor types. However, the analysis does not refer to a specific clone named HK2.1. Details include mutation types, survival impact, and structural mapping of the HK2 protein.
• hK2 (human Kallikrein 2) is investigated as a cancer biomarker and therapeutic target, especially in prostate cancer. Studies confirm robust and homogeneous hK2 expression in non-metastatic and metastatic disease, with promising antitumor responses to hK2-targeting agents. Again, there is no mention of a clone designated HK2.1.

Additional information:

• If "clone HK2.1" refers to a particular monoclonal antibody, cell line, or engineered clone used in research, the current search does not provide relevant results. The search results focus broadly on HK2 enzyme and hK2 protein research, not on specific clones.
• "Clone journal" also appears in the search, but that term refers to fraudulent journals, not biological reagents or research tools.

If your query pertains to a specific scientific reagent (antibody, cell line, etc.), more precise details—such as its catalog number, application, or targeted molecule—are needed to locate relevant scientific citations. If further clarification or context is provided, more targeted findings can be synthesized.