Anti-Mouse DCC – Biotin
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse DCC (Accession # P70211) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829945 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse DCC this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse DCC is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:D337. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse Deleted in Colorectal Cancer (DCC) recognizes Mouse DCC. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Deleted in Colorectal Carcinoma, also known as DCC is a tumor suppressor gene found in axons of the central and peripheral nervous system and in differentiated cell types of the intestine.1 DCC may participate in signaling pathways that regulate cell proliferation and/or differentiation, two cellular processes that often go awry during tumorigenesis.2 DCC is required for the formation of major axonal projections by embryonic cortical neurons, including the corpus callosum, hippocampal commissure, and cortico-thalamic tracts.3 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Vogelstein, B. et al. (1994) Genes Dev. 8: 1174 2. Fearon, ER. et al. (1995) Curr Opin Genet Dev. 5: 72 3. Kennedy, TE. et al. (2008) J Neurochem. 107: 398 Technical Protocols |
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