Sf 21-Derived Recombinant Mouse DCC Extracellular Domain
<0.1 EU/µg as determined by the LAL method
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse DCC this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse DCC is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Blocking: Approximately 10 - 20 μg/mL of the antibody will block 50% of the binding of 50 ng/mL of rcNetrin-2 to immobilized rmDCC/Fc chimera (100 μL of a 2 μg/mL solution was coated in each well) in a functional ELISA assay.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Mouse Deleted in Colorectal Cancer (DCC) recognizes Mouse DCC. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Deleted in Colorectal Carcinoma, also known as DCC is a tumor suppressor gene found in axons of the central and peripheral nervous system and in differentiated cell types of the intestine.1 DCC may participate in signaling pathways that regulate cell proliferation and/or differentiation, two cellular processes that often go awry during tumorigenesis.2 DCC is required for the formation of major axonal projections by embryonic cortical neurons, including the corpus callosum, hippocampal commissure, and cortico-thalamic tracts.3
NCBI Gene Bank ID
References & Citations
1. Vogelstein, B. et al. (1994) Genes Dev. 8: 1174
2. Fearon, ER. et al. (1995) Curr Opin Genet Dev. 5: 72
3. Kennedy, TE. et al. (2008) J Neurochem. 107: 398
Products are for research use only. Not for use in diagnostic or therapeutic procedures.